Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways.
Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways.
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DOI：
10.3389/fpls.2022.968780
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
在处理人类需要的行业，如调味料、香料、颜料、制药和用于生物燃料的生物质时，发展植物类异戊二烯生产至关重要。甲基赤藓糖醇磷酸(MEP)和甲氧戊酸(MVA)的植物途径有助于异戊二烯类化合物的动态生产。然而，MVA和MEP在类异戊二烯生物合成中的串扰还没有完全被认识到。针对3-羟基-3-甲基戊二酰辅酶A还原异构酶(HMGR)和1-脱氧-D-木酮糖-5-磷酸还原异构酶(DXR)在MEP途径中的限速步骤，以及通过催化3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)在MVA途径中的限速步骤，分析了三果杨HMGR的特性和功能。结果表明，与NT杨相比，PtHMGR过表达基因(OES)表现出不同的MEP和MVA相关基因表达。PtDXR过表达上调了MEP相关基因，下调了MVA相关基因。PtDXR和PtHMGR的过表达影响了MVA和MEP途径中类异戊二烯的产生。在这里，结果表明，PtHMGR和PtDXR在调节MEP和MVA相关基因和衍生的异戊二烯类化合物方面发挥着重要作用。这项研究阐明了MVA和MEP通路之间的串扰。它证明了HMGR和DXR在这种串扰中的关键功能，这对调节杨树类异戊二烯的生物合成具有重要意义。
It is critical to develop plant isoprenoid production when dealing with human-demanded industries such as flavoring, aroma, pigment, pharmaceuticals, and biomass used for biofuels. The methylerythritol phosphate (MEP) and mevalonic acid (MVA) plant pathways contribute to the dynamic production of isoprenoid compounds. Still, the cross-talk between MVA and MEP in isoprenoid biosynthesis is not quite recognized. Regarding the rate-limiting steps in the MEP pathway through catalyzing 1-deoxy-D-xylulose5-phosphate synthase and 1-deoxy-D-xylulose5-phosphate reductoisomerase (DXR) and also the rate-limiting step in the MVA pathway through catalyzing 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), the characterization and function of HMGR from Populus trichocarpa (PtHMGR) were analyzed. The results indicated that PtHMGR overexpressors (OEs) displayed various MEP and MVA-related gene expressions compared to NT poplars. The overexpression of PtDXR upregulated MEP-related genes and downregulated MVA-related genes. The overexpression of PtDXR and PtHMGR affected the isoprenoid production involved in both MVA and MEP pathways. Here, results illustrated that the PtHMGR and PtDXR play significant roles in regulating MEP and MVA-related genes and derived isoprenoids. This study clarifies cross-talk between MVA and MEP pathways. It demonstrates the key functions of HMGR and DXR in this cross-talk, which significantly contribute to regulate isoprenoid biosynthesis in poplars.
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