A dual-mode mobile phone microscope using the onboard camera flash and ambient light.

A dual-mode mobile phone microscope using the onboard camera flash and ambient light.
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DOI:
10.1038/s41598-018-21543-2
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发表时间:
2018-02-19
期刊:
影响因子:
4.6
通讯作者:
Gibson BC
Gibson BC
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Orth A;Wilson ER;Thompson JG;Gibson BC

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手机显微镜是即时成像的天然平台,但目前的解决方案需要外部供电的照明光源,从而增加了体积和成本。我们提出了一种使用内部闪光灯或阳光作为照明源的手机显微镜,从而在保持功能和性能的同时降低了复杂性。该显微镜具有明场和暗场成像模式,能够对从植物到哺乳动物细胞的样品进行显微镜观察。我们描述了显微镜的设计原理,组装过程,并通过未标记细胞核的可视化来观察牛精子和浮游动物的运动,展示了其成像能力。
Mobile phone microscopes are a natural platform for point-of-care imaging, but current solutions require an externally powered illumination source, thereby adding bulk and cost. We present a mobile phone microscope that uses the internal flash or sunlight as the illumination source, thereby reducing complexity whilst maintaining functionality and performance. The microscope is capable of both brightfield and darkfield imaging modes, enabling microscopic visualisation of samples ranging from plant to mammalian cells. We describe the microscope design principles, assembly process, and demonstrate its imaging capabilities through the visualisation of unlabelled cell nuclei to observing the motility of cattle sperm and zooplankton.
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