Deep optoacoustic localization microangiography of ischemic stroke in mice.
Deep optoacoustic localization microangiography of ischemic stroke in mice.
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小鼠缺血性卒中的深光声定位微血管造影术。
DOI：
10.1038/s41467-023-39069-1
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发表时间：
2023-06-16
影响因子：
16.6
通讯作者：
Razansky, Daniel
中科院分区：
文献类型：
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作者：
Dean-Ben, Xose Luis;Robin, Justine;Nozdriukhin, Daniil;Ni, Ruiqing;Zhao, Jim;Gluck, Chaim;Droux, Jeanne;Sendon-Lago, Juan;Chen, Zhenyue;Zhou, Quanyu;Weber, Bruno;Wegener, Susanne;Vidal, Anxo;Arand, Michael;El Amki, Mohamad;Razansky, Daniel
迄今为止，哺乳动物组织深处微血管结构的超分辨率光声成像一直受到密集红细胞的强烈吸收的阻碍。在这里，我们设计了 5μm 生物相容性二氯甲烷基微滴，在近红外波长下表现出比红细胞高几个数量级的光吸收，从而实现体内单颗粒检测。我们展示了小鼠大脑的非侵入性三维微血管造影超出了声学衍射极限（<20μm 分辨率）。还完成了微血管网络中的血流速度量化和光通量映射。在患有急性缺血性中风的小鼠中，通过超分辨率和光谱光声成像实现的多参数多尺度观察揭示了同侧和对侧大脑半球的微血管密度、流量和氧饱和度的显着差异。鉴于光声学对活组织中的功能、代谢和分子事件的敏感性，新方法为具有无与伦比的分辨率、对比度和速度的非侵入性显微观察铺平了道路。毫米级深度的光声超分辨率受到血细胞强烈背景吸收的阻碍。在这里，作者使用具有高光吸收率的二氯甲烷微滴，并通过光声定位演示了小鼠大脑的 3D 微血管造影。
Super-resolution optoacoustic imaging of microvascular structures deep in mammalian tissues has so far been impeded by strong absorption from densely-packed red blood cells. Here we devised 5 µm biocompatible dichloromethane-based microdroplets exhibiting several orders of magnitude higher optical absorption than red blood cells at near-infrared wavelengths, thus enabling single-particle detection in vivo. We demonstrate non-invasive three-dimensional microangiography of the mouse brain beyond the acoustic diffraction limit (<20 µm resolution). Blood flow velocity quantification in microvascular networks and light fluence mapping was also accomplished. In mice affected by acute ischemic stroke, the multi-parametric multi-scale observations enabled by super-resolution and spectroscopic optoacoustic imaging revealed significant differences in microvascular density, flow and oxygen saturation in ipsi- and contra-lateral brain hemispheres. Given the sensitivity of optoacoustics to functional, metabolic and molecular events in living tissues, the new approach paves the way for non-invasive microscopic observations with unrivaled resolution, contrast and speed. Optoacoustic super-resolution at millimeter-scale depths has been impeded by the strong background absorption from blood cells. Here, the authors use dichloromethane microdroplets with high optical absorption and demonstrate 3D microangiography of the mouse brain via optoacoustic localization.
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影响因子：
8.8
作者：
El Amki, Mohamad;Glueck, Chaim;Wegener, Susanne
通讯作者：
Wegener, Susanne
影响因子：
7.9
作者：
Dean-Ben, Xose Luis;Razansky, Daniel
通讯作者：
Razansky, Daniel
影响因子：
10.6
作者：
Demene, Charlie;Deffieux, Thomas;Tanter, Mickael
通讯作者：
Tanter, Mickael
影响因子：
3.6
作者：
Dean-Ben, X. Luis;Ding, Lu;Razansky, Daniel
通讯作者：
Razansky, Daniel
DOI：
10.1007/978-94-007-7678-4_27
发表时间：
2016-01-01
期刊：
BIOMARKERS IN CARDIOVASCULAR DISEASE, VOLS 1 AND 2
影响因子：
--
作者：
Derosa, Giuseppe;Maffioli, Pamela
通讯作者：
Maffioli, Pamela