Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis.
Comparative transcriptome analysis coupled to X-ray CT reveals sucrose supply and growth velocity as major determinants of potato tuber starch biosynthesis.
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DOI：
10.1186/1471-2164-11-93
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发表时间：
2010-02-05
期刊：
影响因子：
4.4
通讯作者：
Sonnewald U
中科院分区：
文献类型：
--
作者：
Ferreira SJ;Senning M;Sonnewald S;Kessling PM;Goldstein R;Sonnewald U
尽管马铃薯块茎淀粉的生物合成过程已被充分了解，但其生物合成的调控机制仍不清楚。转录组分析提供了关于基因如何调控的有价值的信息。因此，本研究旨在研究马铃薯叶片和块茎中淀粉生物合成基因在不同条件下的转录调控。更具体地说，我们每天在叶片和块茎中，在块茎诱导过程中，以及在以不同速度生长的块茎中观察基因表达。为了测定马铃薯块茎的生长速度，建立了一种基于X射线计算机断层扫描（X-ray CT）的新方法。叶片和块茎之间的比较转录组分析显示，在两种组织中差异表达的相同基因具有惊人的相似性。在块茎中，观察到颗粒结合淀粉合成酶（GBSS）表达的振荡，这可能与来源叶片的蔗糖供应有关。X射线CT用于确定块茎体积随时间的变化，并计算生长速度。虽然生长速度与淀粉合成基因的表达量之间不存在线性相关，但在生长期和非生长期块茎之间存在显著差异。共表达分析用于鉴定与淀粉生物合成基因正相关的可能调控淀粉生物合成的转录因子。大多数淀粉生物合成酶由基因家族编码。共表达分析表明，这些基因家族的相同成员在叶片和块茎中受到共调控。这表明，在叶和块茎中的暂时性和储存淀粉生物合成的调节，分别是惊人的相似。X射线CT可用于监测地下器官的生长和发育，并允许将块茎生长与基因表达的变化联系起来。比较转录组分析提供了一个有用的工具，以确定可能参与调节淀粉生物合成的转录因子。
Even though the process of potato tuber starch biosynthesis is well understood, mechanisms regulating biosynthesis are still unclear. Transcriptome analysis provides valuable information as to how genes are regulated. Therefore, this work aimed at investigating transcriptional regulation of starch biosynthetic genes in leaves and tubers of potato plants under various conditions. More specifically we looked at gene expression diurnally in leaves and tubers, during tuber induction and in tubers growing at different velocities. To determine velocity of potato tuber growth a new method based on X-ray Computed Tomography (X-ray CT) was established. Comparative transcriptome analysis between leaves and tubers revealed striking similarities with the same genes being differentially expressed in both tissues. In tubers, oscillation of granule bound starch synthase (GBSS) expression) was observed which could be linked to sucrose supply from source leaves. X-ray CT was used to determine time-dependent changes in tuber volume and the growth velocity was calculated. Although there is not a linear correlation between growth velocity and expression of starch biosynthetic genes, there are significant differences between growing and non-growing tubers. Co-expression analysis was used to identify transcription factors positively correlating with starch biosynthetic genes possibly regulating starch biosynthesis. Most starch biosynthetic enzymes are encoded by gene families. Co-expression analysis revealed that the same members of these gene families are co-regulated in leaves and tubers. This suggests that regulation of transitory and storage starch biosynthesis in leaves and tubers, respectively, is surprisingly similar. X-ray CT can be used to monitor growth and development of belowground organs and allows to link tuber growth to changes in gene expression. Comparative transcriptome analysis provides a useful tool to identify transcription factors possibly involved in the regulation of starch biosynthesis.
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影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
DOI：
10.1016/j.nima.2008.03.016
发表时间：
2008-06-11
影响因子：
1.4
作者：
Hanke, Randolf;Fuchs, Theobald;Uhlmann, Norman
通讯作者：
Uhlmann, Norman
影响因子：
7.2
作者：
Geigenberger, P;Stitt, M
通讯作者：
Stitt, M
影响因子：
5.4
作者：
HOVENKAMPHERMELINK, JHM;JACOBSEN, E;FEENSTRA, WJ
通讯作者：
FEENSTRA, WJ
影响因子：
7.4
作者：
Mérida, A;Rodríguez-Galán, JM;Romero, JM
通讯作者：
Romero, JM