Genome-Wide Analysis of the Peroxidase Gene Family and Verification of Lignin Synthesis-Related Genes in Watermelon.
Genome-Wide Analysis of the Peroxidase Gene Family and Verification of Lignin Synthesis-Related Genes in Watermelon.
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西瓜过氧化物酶基因家族的全基因组分析及木质素合成相关基因的验证
DOI：
10.3390/ijms23020642
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发表时间：
2022-01-07
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Yang T;Zhang P;Pan J;Amanullah S;Luan F;Han W;Liu H;Wang X
西瓜（Citrullus lanatus）是世界上重要的园艺作物，但由于果皮硬度过高而造成的果皮开裂严重影响了西瓜的品质。木质化是Ⅲ类过氧化物酶（PRX）的重要功能之一，其在植物细胞壁中的积累导致细胞增厚和木材硬化。为了深入了解果皮硬度与木质素积累之间的关系以及PRXs影响果皮木质素生物合成的作用，我们使用全基因组生物信息学分析研究了果皮硬度与木质素积累之间的关系。结果表明，果皮中木质素积累逐渐增多，形成石细胞结构，组织木质化导致果皮硬度增加。利用生物信息学分析，共鉴定出79个ClPRX（III类），它们广泛分布在11条染色体上。结果表明，ClPRXs可分为7类11个亚类，每个亚类的基因成员具有高度保守的内含子结构。重复模式分析表明，在ClPRX扩增过程中发生了缺失和复制事件。然而，在全蛋白序列比对分析中，没有观察到高同源性，虽然都包含四个保守的功能位点。重复模式分析表明，在ClPRXs的扩增过程中发生了缺失和复制事件。预测的启动子顺式作用元件和qRT-PCR分析在四个组织（叶，叶柄，茎，果皮）显示不同的表达模式的组织特异性，非生物胁迫和激素反应提供了遗传基础的ClPRX基因家族参与在植物中的各种生理过程。据我们所知，我们首次报道了两个ClPRX在西瓜皮木质素合成中的关键作用。总之，本研究中收集的大量数据可用于ClPRXs在西瓜生长发育以及激素和非生物胁迫响应中的额外功能分析。
Watermelon (Citrullus lanatus) is an important horticultural crop worldwide, but peel cracking caused by peel hardness severely decreases its quality. Lignification is one of the important functions of class III peroxidase (PRX), and its accumulation in the plant cell wall leads to cell thickening and wood hardening. For in-depth physiological and genetical understanding, we studied the relationship between peel hardness and lignin accumulation and the role of PRXs affecting peel lignin biosynthesis using genome-wide bioinformatics analysis. The obtained results showed that lignin accumulation gradually increased to form the peel stone cell structure, and tissue lignification led to peel hardness. A total of 79 ClPRXs (class III) were identified using bioinformatics analysis, which were widely distributed on 11 chromosomes. The constructed phylogenetics indicated that ClPRXs were divided into seven groups and eleven subclasses, and gene members of each group had highly conserved intron structures. Repeated pattern analysis showed that deletion and replication events occurred during the process of ClPRX amplification. However, in the whole-protein sequence alignment analysis, high homology was not observed, although all contained four conserved functional sites. Repeated pattern analysis showed that deletion and replication events occurred during ClPRXs’ amplification process. The prediction of the promoter cis-acting element and qRT-PCR analysis in four tissues (leaf, petiole, stem, and peel) showed different expression patterns for tissue specificity, abiotic stress, and hormone response by providing a genetic basis of the ClPRX gene family involved in a variety of physiological processes in plants. To our knowledge, we for the first time report the key roles of two ClPRXs in watermelon peel lignin synthesis. In conclusion, the extensive data collected in this study can be used for additional functional analysis of ClPRXs in watermelon growth and development and hormone and abiotic stress response.
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影响因子：
4.3
作者：
Fang, Xufeng;Liu, Shi;Dai, Zuyun
通讯作者：
Dai, Zuyun
影响因子：
4.3
作者：
Herrero, Joaquin;Fernandez-Perez, Francisco;Miguel Zapata, Jose
通讯作者：
Miguel Zapata, Jose
影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
2.7
作者：
Armanini, D;Nacamulli, D;Fiore, C
通讯作者：
Fiore, C
DOI：
10.1073/pnas.2535513100
发表时间：
2003-12-23
影响因子：
11.1
作者：
Moore, RC;Purugganan, MD
通讯作者：
Purugganan, MD