Induction of long-lived germinal centers associated with persisting antigen after viral infection.
Induction of long-lived germinal centers associated with persisting antigen after viral infection.
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与病毒感染后持续的抗原相关的长寿命生发中心的诱导。
DOI：
10.1084/jem.183.5.2259
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发表时间：
1996-05-01
期刊：
影响因子：
--
通讯作者：
Zinkernagel RM
中科院分区：
文献类型：
--
作者：
Bachmann MF;Odermatt B;Hengartner H;Zinkernagel RM
水泡性口炎病毒（VSV）诱导早期不依赖于T细胞的中和IgM反应，随后是长期的、T细胞依赖的IgG反应。我们使用数100倍的VSV病毒粒子的特异性扩增因子进行免疫组织学分析，以分析免疫后不同时间点VSV特异性B细胞的定位。在 IgM 反应高峰期（第 4 天），VSV 特异性 B 细胞主要存在于红髓和边缘区，但不存在于 T 区。这些 B 细胞主要在细胞质中被染色，这表明它们是抗体分泌细胞。免疫后第 6 天，含有表面染色的 VSV 特异性 B 细胞的生发中心 (GC) 变得可检测到，并在第 12 天完全建立。同时，红髓中存在大的 VSV 特异性 B 细胞聚集体。与持续抗原相关的大量 VSV 特异性 GC 在免疫后 1 个月及之后出现，即比半抗原观察到的时间要长得多。免疫后 100 天仍可检测到一些 GC，其表现出滤泡树突状细胞并含有 VSV 特异性、增殖的 B 细胞。在没有佐剂的情况下用重组VSV-糖蛋白进行免疫后也观察到了长寿命的GC。因此，一些抗病毒保护性（记忆）B细胞在与持续抗原相关的长寿命GC中循环并局部增殖，因此似乎负责长期维持升高的抗体水平。这些观察结果扩展了佐剂库中载体半抗原抗原或与特定 IgG 复合的早期研究；他们首次展示了抗原和特定记忆 B 细胞的共定位，并分析了针对急性病毒感染的保护性中和抗体反应。
Vesicular stomatitis virus (VSV) induces an early T cell-independent neutralizing lgM response that is followed by a long-lived, T cell- dependent lgG response. We used the specific amplification factor of several 100x of VSV-virions for immunohistology to analyze the localization of VSV-specific B cells at different time points after immunization. At the peak of the IgM response (day 4), VSV-specific B cells were predominantly present in the red pulp and marginal zone but not in the T area. These B cells were mostly stained in the cytoplasm, characterizing them as antibody secreting cells. By day 6 after immunization, germinal centers (GC) containing surface-stained VSV- specific B cells became detectable and were fully established by day 12. At the same time, large VSV-specific B cell aggregates were present in the red pulp. High numbers of VSV-specific GC associated with persisting antigen were present 1 mo after immunization and later, i.e., considerably longer than has been observed for haptens. Some GC, exhibiting follicular dendritic cells and containing VSV-specific, proliferating B cells were still detectable up to 100 d after immunization. Long-lived GC were also observed after immunization with recombinant VSV-glycoprotein in absence of adjuvants. Thus some anti- virally protective (memory) B cells are cycling and locally proliferate in long-lived GC in association with persisting antigen and therefore seem responsible for long-term maintenance of elevated antibody levels. These observations extend earlier studies with carrier hapten antigens in adjuvant depots or complexed with specific IgG; they are the first to show colocalization of antigen and specific memory B cells and to analyze a protective neutralizing antibody response against an acute viral infection.
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影响因子：
5.4
作者：
LIU, YJ;OLDFIELD, S;MACLENNAN, ICM
通讯作者：
MACLENNAN, ICM
影响因子：
56.9
作者：
ARPIN, C;DECHANET, J;LIU, YJ
通讯作者：
LIU, YJ
影响因子：
4.3
作者：
Fehr, T;Bachmann, MF;Zinkernagel, RM
通讯作者：
Zinkernagel, RM
影响因子：
64.8
作者：
LAU, LL;JAMIESON, BD;AHMED, R
通讯作者：
AHMED, R
影响因子：
3.1
作者：
HECHT, TT;PAUL, WE
通讯作者：
PAUL, WE