Development and growth of organs in living whole embryo and larval grafts in zebrafish.
Development and growth of organs in living whole embryo and larval grafts in zebrafish.
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DOI：
10.1038/s41598-017-16642-5
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发表时间：
2017-11-28
影响因子：
4.6
通讯作者：
Sakai N
中科院分区：
文献类型：
--
作者：
Kawasaki T;Maeno A;Shiroishi T;Sakai N
与年龄相关的全身环境影响异时性共生伴侣的神经发生和器官再生；然而，操纵小胚胎的困难使得无法分析老化的系统环境对发育阶段原始器官的影响。在这里，我们描述了一种新型移植系统，通过将宿主血管侵入移植物来支持整个活胚胎/幼虫作为免疫缺陷斑马鱼的移植物，从而允许通过皮下移植产生类似于异时性联体共生的身体。尽管移植的胚胎/幼虫形成了大多数器官，但并非所有器官发生都得到同等支持；虽然大脑、眼睛和肠道通常发育，但肝脏、睾丸和心脏发育不足，甚至偶尔消失。宿主生殖细胞的去除刺激了移植胚胎中睾丸的发育。这些结果表明原始睾丸容易受到源自老年宿主生殖细胞的全身环境的影响，并暗示原始肝脏和心脏是相似的。将这种方法应用于胚胎致死突变体后，从突变体中获得了各种类型的器官，包括在去除生殖细胞的受体中发育的睾丸，以及可存活的后代。这种独特的移植系统将带来对与年龄相关的系统环境的新见解，这对于脊椎动物的器官发生至关重要。
Age-related systemic environments influence neurogenesis and organ regeneration of heterochronic parabiotic partners; however, the difficulty of manipulating small embryos prevents the effects of aged systemic environments on primitive organs at the developmental stage from being analysed. Here, we describe a novel transplantation system to support whole living embryos/larvae as grafts in immunodeficient zebrafish by the intrusion of host blood vessels into the grafts, allowing bodies similar to those of heterochronic parabiosis to be generated by subcutaneous grafting. Although grafted embryos/larvae formed most organs, not all organogenesis was supported equally; although the brain, eyes and the intestine usually developed, the liver, testes and heart developed insufficiently or even occasionally disappeared. Removal of host germ cells stimulated testis development in grafted embryos. These results indicate that primitive testes are susceptible to the systemic environments that originated from the germ cells of aged hosts and imply that the primitive liver and heart are similar. Upon applying this method to embryonic lethal mutants, various types of organs, including testes that developed in germ-cell-removed recipients, and viable offspring were obtained from the mutants. This unique transplantation system will lead to new insights into the age-related systemic environments that are crucial for organogenesis in vertebrates.
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影响因子：
2.6
作者：
Korzh, S;Emelyanov, A;Korzh, V
通讯作者：
Korzh, V
影响因子：
64.8
作者：
Conboy, IM;Conboy, MJ;Rando, TA
通讯作者：
Rando, TA
影响因子：
64.5
作者：
Rottbauer, W;Saurin, AJ;Fishman, M
通讯作者：
Fishman, M
影响因子：
4.1
作者：
Rétaux S;Casane D
通讯作者：
Casane D
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF