Microvillous cells in the olfactory epithelium express elements of the solitary chemosensory cell transduction signaling cascade.
Microvillous cells in the olfactory epithelium express elements of the solitary chemosensory cell transduction signaling cascade.
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DOI：
10.1371/journal.pone.0202754
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Tizzano M
中科院分区：
文献类型：
--
作者：
Genovese F;Tizzano M
鼻腔内有一系列化学反应细胞，包括嗅觉系统和其他一些参与刺激物检测和反应的细胞。对刺激物和细菌做出反应的孤立化学感应细胞 (SCC) 表达瞬时受体电位通道 TRPM5，这是味觉转导信号级联的重要元件。微绒毛细胞（MVC）是位于主嗅上皮顶层的非神经元细胞，也表达 TRPM5，但其功能尚未阐明。 TRPM5 阳性 MVC 与 SCC 一样，显示出表达胆碱乙酰转移酶 (ChAT) 的胆碱能表型，但无法检测到苦味转导级联的其他元件。我们使用更灵敏的基因表达和染色技术重新检查了 TRPM5 阳性 MVC，以阐明它们是否仅依赖于 TRPM5 和 ChAT，还是表达味觉/SCC 转导级联的其他元件。通过分析整个嗅粘膜和分离的嗅觉感觉神经元的现有 RNA 测序数据，我们确定味觉/SCC 转导级联的几个元件（包括味觉受体）在嗅觉粘膜中除嗅觉感觉神经元以外的细胞中表达。免疫染色证实嗅粘膜细胞亚群中存在 TRPM5 和 ChAT，还显示 PLCB2、gustducin 和 T1R3 的表达。具体来说，这些细胞被鉴定为 TRPM5 阳性 MVC。此外，我们检查了 MVC 是否由三叉神经纤维支配，类似于 SCC。使用针对三叉神经标志物降钙素基因相关肽和 P 物质的抗体，我们确定，尽管具有胆碱能表型，但嗅粘膜中的大多数 MVC 缺乏一致的三叉神经支配。我们的研究结果表明，MVC 与 SCC 一样，表达苦味转导级联的所有元素，但与 SCC 不同，它们仅具有稀疏的三叉神经支配。 MVC 的胆碱能表型表明周围嗅觉上皮通过释放乙酰胆碱具有调节功能。
The nasal cavity hosts an array of chemoresponsive cells, including the extended olfactory system and several other cells involved in detection of and responses to irritants. Solitary chemosensory cells (SCCs), which respond to irritants and bacteria, express the transient receptor potential channel TRPM5 an essential element of the taste transduction-signaling cascade. Microvillous cells (MVCs), non-neuronal cells situated in the apical layer of the main olfactory epithelium, also express TRPM5, but their function has not yet been clarified. TRPM5-positive MVCs, like SCCs, show a cholinergic phenotype expressing choline acetyl transferase (ChAT), but none of the other elements of the bitter taste transduction cascade could be detected. We reexamined TRPM5-positive MVCs with more sensitive gene expression and staining techniques to clarify whether they rely only on TRPM5 and ChAT or express other elements of the taste/SCC transduction cascade. Analyzing existing RNA sequencing data from whole olfactory mucosa and isolated olfactory sensory neurons, we determined that several elements of the taste/SCC transduction cascade, including taste receptors, are expressed in the olfactory mucosa in cells other than olfactory sensory neurons. Immunostaining confirmed the presence TRPM5 and ChAT in a subset of cells of the olfactory mucosa, which also showed the expression of PLCB2, gustducin, and T1R3. Specifically, these cells were identified as TRPM5-positive MVCs. Furthermore, we examined whether MVCs are innervated by trigeminal fibers, similarly to SCCs. Using antibodies against trigeminal nerve markers calcitonin gene-related peptide and substance P, we determined that, despite the cholinergic phenotype, most MVCs in the olfactory mucosa lacked consistent trigeminal innervation. Our findings indicate that MVCs, like SCCs, express all the elements of the bitter taste transduction cascade but that, unlike SCCs, they possess only sparse trigeminal innervation. The cholinergic phenotype of MVCs suggests a modulatory function of the surrounding olfactory epithelium, through the release of acetylcholine.
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影响因子：
5.5
作者：
Dando, Robin;Roper, Stephen D.
通讯作者：
Roper, Stephen D.
DOI：
10.1007/s11068-005-5051-y
发表时间：
2005-03-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Gulbransen, BD;Finger, TE
通讯作者：
Finger, TE
影响因子：
2.4
作者：
Hansen, Anne;Finger, Thomas E.
通讯作者：
Finger, Thomas E.
影响因子：
5.4
作者：
Clapp, TR;Medler, KF;Kinnamon, SC
通讯作者：
Kinnamon, SC
TRPM5是一种味觉信号的瞬态受体电位离子通道，是化学感应细胞中普遍存在的信号传导成分。
影响因子：
2.4
作者：
Kaske, Silke;Krasteva, Gabriele;Koenig, Peter;Kummer, Wolfgang;Hofmann, Thomas;Gudermann, Thomas;Chubanov, Vladimir
通讯作者：
Chubanov, Vladimir