Karyotype analysis and genetic variation of a mutant in Siraitia grosvenorii.
Karyotype analysis and genetic variation of a mutant in Siraitia grosvenorii.
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罗汉果突变体的核型分析及遗传变异
DOI：
10.1007/s11033-011-0855-6
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
Mo C
中科院分区：
文献类型：
--
作者：
Fu W;Ma X;Tang Q;Mo C
本研究分析了罗汉果突变体与野生型的核型差异和遗传变异。遗传变异包括基因组的变化和SRAP分子标记的基因表达。结果表明，野生型S.罗汉果为二倍体，染色体数目为2n = 2x = 28，而突变体为四倍体，染色体数目为2n = 4x = 56。189对引物组合共扩增出4573条DNA带，其中多态性引物577对，平均每对引物扩增出3.1条带，相同引物1998对。大多数引物对扩增出的条带差异不明显。在比较二倍体和四倍体品系后，数据普遍表明多态性将相当低。用133对引物组合共扩增出2917条带，筛选出稳定且差异明显的片段，并进行克隆和测序。对92个差异表达片段进行了测序。序列分析表明，大部分片段与1，5-二磷酸核酮糖羧化酶/加氧酶、磷酸烯醇式丙酮酸羧激酶、丙酮酸激酶、过氧化物酶体膜转运蛋白、NBS-LRR型抗性蛋白、蛋白磷酸酶等抗逆基因和光合作用基因具有显著的同源性。
This study analyzed the differences in karyotype and genetic variation between a mutant and wild-type Siraitia grosvenorii. Genetic variation included changes in genome and gene expression by SRAP molecular markers. Results showed that wild-type S. grosvenorii was diploid, with a chromosome number of 2n = 2x = 28, whereas the mutant was tetraploid with a chromosome number of 2n = 4x = 56. 4573 DNA bands were obtained using 189 different primer combinations, 577 of which were polymorphic, averaging 3.1 bands for each primer pair, while 1998 pairs were identical. There were no apparent differences on bands amplified by most primer pairs. After comparing the diploid and tetraploid strains, the data generally indicated that the polymorphism would be quite low. 2917 cDNA bands were generated using 133 primer combinations, and stable and clearly differential fragments were sorted out, cloned and sequenced. Ninety-two differentially expressed fragments were successfully sequenced. Sequence analysis showed that most fragments had significant homologous nucleotide sequences with resistant to stress and photosynthesis genes, including ribulose-1,5-bisphosphate carboxylase/oxygenase, phosphoenolpyruvate carboxykinase, pyruvate kinase, peroxisomal membrane transporter, NBS-LRR type resistance protein, protein phosphatase and others.
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影响因子：
3.1
作者：
Ma, Xue-Feng;Gustafson, J. Perry
通讯作者：
Gustafson, J. Perry
影响因子：
9.4
作者：
Pignatta, Daniela;Dilkes, Brian P.;Comai, Luca
通讯作者：
Comai, Luca
影响因子：
5.4
作者：
Li, G;Quiros, CF
通讯作者：
Quiros, CF
影响因子：
2.8
作者：
Luo, Liting;Zhang, Jinrui;He, Guangyuan
通讯作者：
He, Guangyuan
影响因子：
4.3
作者：
Qin, X.;Xiaojian, S.;Heimbach, J.
通讯作者：
Heimbach, J.