P2CS: updates of the prokaryotic two-component systems database.
P2CS: updates of the prokaryotic two-component systems database.
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DOI：
10.1093/nar/gku968
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发表时间：
2015-01
影响因子：
14.9
通讯作者：
Barakat M
中科院分区：
文献类型：
--
作者：
Ortet P;Whitworth DE;Santaella C;Achouak W;Barakat M
P2CS数据库（http：//www.p2cs.org/）是用于分析P2CS双组分系统（TCS）的综合资源。TCS由受体组氨酸激酶（HK）和伴侣反应调节因子（RR）组成，并控制重要的原核生物行为。P2 CS的最新版本包括来自2758个测序的原核基因组的164 651个TCS蛋白质。自从上次描述P2CS以来，它增加了几个重要的新特性。用户可以通过BLAST搜索P2CS，将命中添加到他们的购物车，同源蛋白质可以使用MUSCLE进行比对，并使用P2CS中的Jalview进行查看。P2CS还提供了基于来自整个基因组的RR和HK的保守信号传导结构域的系统发育树。HK和RR树的基因组织和结构域的架构进行了注释，提供了当代基因集的进化起源的见解。大多数TCS由相邻的HK和RR基因编码，然而，“孤儿”未配对的TCS基因也很丰富，鉴定它们的伴侣蛋白是具有挑战性的。P2CS现在提供配对的HK和RR树，其中蛋白质来自所示的相同遗传基因座。这允许评估整个TCS的进化关系，在某些情况下，还可以鉴定孤儿TCS蛋白的候选伴侣。
The P2CS database (http://www.p2cs.org/) is a comprehensive resource for the analysis of Prokaryotic Two-Component Systems (TCSs). TCSs are comprised of a receptor histidine kinase (HK) and a partner response regulator (RR) and control important prokaryotic behaviors. The latest incarnation of P2CS includes 164 651 TCS proteins, from 2758 sequenced prokaryotic genomes. Several important new features have been added to P2CS since it was last described. Users can search P2CS via BLAST, adding hits to their cart, and homologous proteins can be aligned using MUSCLE and viewed using Jalview within P2CS. P2CS also provides phylogenetic trees based on the conserved signaling domains of the RRs and HKs from entire genomes. HK and RR trees are annotated with gene organization and domain architecture, providing insights into the evolutionary origin of the contemporary gene set. The majority of TCSs are encoded by adjacent HK and RR genes, however, ‘orphan’ unpaired TCS genes are also abundant and identifying their partner proteins is challenging. P2CS now provides paired HK and RR trees with proteins from the same genetic locus indicated. This allows the appraisal of evolutionary relationships across entire TCSs and in some cases the identification of candidate partners for orphan TCS proteins.
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影响因子：
5.8
作者：
Waterhouse, Andrew M.;Procter, James B.;Barton, Geoffrey J.
通讯作者：
Barton, Geoffrey J.
影响因子：
4.4
作者：
Barakat M;Ortet P;Whitworth DE
通讯作者：
Whitworth DE
影响因子：
3
作者：
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通讯作者：
Maréchal E
影响因子：
5.4
作者：
Wuichet, Kristin;Cantwell, Brian J.;Zhulin, Igor B.
通讯作者：
Zhulin, Igor B.
影响因子：
2.8
作者：
de Been, Mark;Francke, Christof;Siezen, Roland J.
通讯作者：
Siezen, Roland J.