Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against IBV and NDV challenge.
Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against IBV and NDV challenge.
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表达新城疫病毒 (NDV) 血凝素神经氨酸酶 (HN) 蛋白的重组传染性支气管炎病毒 (IBV) H120 疫苗株可保护鸡免受 IBV 和 NDV 攻击
DOI：
10.1007/s00705-016-2764-4
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发表时间：
2016-05
影响因子：
2.7
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Yang X;Zhou Y;Li J;Fu L;Ji G;Zeng F;Zhou L;Gao W;Wang H
传染性支气管炎（IB）和纽卡斯尔疫（ND）是鸡的常见病毒性疾病，分别由传染性支气管炎病毒（IBV）和纽卡斯尔疫病毒（NDV）引起。用IBV-H120和NDV-LaSota弱毒株免疫对控制IB和ND具有重要意义。然而，传统的减毒活疫苗价格昂贵，并且导致无法区分感染的鸡和接种的鸡。因此，迫切需要开发新的有效疫苗。在本研究中，我们利用先前建立的反向遗传学系统，产生了一个重组IBV病毒的基础上，IBV H120疫苗株表达的血凝素-神经氨酸酶（HN）蛋白的NDV。重组病毒R-H120-HN/5a的生长动力学、致病性和病毒滴度与亲本IBV H120相似，但具有NDV的血凝活性。用R-H120-HN/5a病毒接种SPF鸡，诱导的体液应答水平与LaSota/H120商业双价疫苗相当，并对强毒IBV和NDV的攻击提供显著保护。综上所述，本研究结果表明IBV H120株可作为设计抗IB和其他传染病疫苗的有效工具，IBV R-H120-HN/5a的产生为开发有效的IBV和NDV双价疫苗奠定了坚实的基础。
Infectious bronchitis (IB) and Newcastle disease (ND) are common viral diseases of chickens, which are caused by infectious bronchitis virus (IBV) and Newcastle disease virus (NDV), respectively. Vaccination with live attenuated strains of IBV-H120 and NDV-LaSota are important for the control of IB and ND. However, conventional live attenuated vaccines are expensive and result in the inability to differentiate between infected and vaccinated chickens. Therefore, there is an urgent need to develop new efficacious vaccines. In this study, using a previously established reverse genetics system, we generated a recombinant IBV virus based on the IBV H120 vaccine strain expressing the haemagglutinin-neuraminidase (HN) protein of NDV. The recombinant virus, R-H120-HN/5a, exhibited growth dynamics, pathogenicity and viral titers that were similar to those of the parental IBV H120, but it had acquired hemagglutination activity from NDV. Vaccination of SPF chickens with the R-H120-HN/5a virus induced a humoral response at a level comparable to that of the LaSota/H120 commercial bivalent vaccine and provided significant protection against challenge with virulent IBV and NDV. In summary, the results of this study indicate that the IBV H120 strain could serve as an effective tool for designing vaccines against IB and other infectious diseases, and the generation of IBV R-H120-HN/5a provides a solid foundation for the development of an effective bivalent vaccine against IBV and NDV.
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DOI：
10.1292/jvms.14-0599
发表时间：
2015-08
期刊：
The Journal of veterinary medical science
影响因子：
--
作者：
Aihara N;Horiuchi N;Hikichi N;Ochiai M;Hosoda Y;Ishikawa Y;Shimazaki Y;Oishi K
通讯作者：
Oishi K
影响因子：
3.7
作者：
Mondal SP;Cardona CJ
通讯作者：
Cardona CJ
影响因子：
3.7
作者：
Bentley K;Armesto M;Britton P
通讯作者：
Britton P
影响因子：
3.7
作者：
Armesto M;Cavanagh D;Britton P
通讯作者：
Britton P
影响因子：
3.3
作者：
Chen L;Zhang T;Han Z;Liang S;Xu Y;Xu Q;Chen Y;Zhao Y;Shao Y;Li H;Wang K;Kong X;Liu S
通讯作者：
Liu S