Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China.
Molecular and antigenic characteristics of Massachusetts genotype infectious bronchitis coronavirus in China.
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DOI：
10.1016/j.vetmic.2015.10.003
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发表时间：
2015-12-31
影响因子：
3.3
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Chen L;Zhang T;Han Z;Liang S;Xu Y;Xu Q;Chen Y;Zhao Y;Shao Y;Li H;Wang K;Kong X;Liu S
31株马萨诸塞州基因型IBV中有24株为H120疫苗株再分离株。31例中仅2例为M41样致病型。马萨诸塞州基因型中的5个源自H120样和其它类型IBV之间的重组事件。尽管S1基因分析表明它们是马萨诸塞州的基因型，但由于分离导致出现了一个新的IBV血清型。从1717个鸡群中分离到418株IBV。29个分离株被归类为马萨诸塞州基因型。进一步研究了这29个分离株以及先前分离的两个马萨诸塞州基因型IBV毒株。经全基因组序列分析，24株为H120型，2株为M41型，表明大部分IBV分离株可能为再分离疫苗病毒。其余5个IBV分离株（ck/CH/LHB/111172、ck/CH/LSD/111219、ck/CH/LHB/130598、ck/CH/LDL/110931和ck/CH/LHB/130573）显示起源于H120样疫苗株与其他类型病毒之间的天然重组事件。病毒交叉中和试验发现，ck/CH/LHB/111172、ck/CH/LSD/111219和ck/CH/LHB/130598的抗原性与H120相似。接种H120疫苗提供了完全的保护，免受这些菌株的挑战。然而，分离株ck/CH/LDL/110931和ck/CH/LHB/130573的血清型与其亲本病毒和本研究中的其他血清型不同。此外，接种H120疫苗不能提供针对这两种分离株攻毒的保护。本研究的结果表明，重组是导致新血清型毒株出现的机制，并且它具有改变病毒血清型的能力。因此，对接种IBV活疫苗的鸡群进行IBV监测，以及考虑使用灭活或/和基因工程疫苗有效控制IBV感染的新策略是非常重要的。
24 out of 31 Massachusetts genotype IBVs are reisolates of H120 vaccine strain. Only 2 out of 31 are of M41-like pathogenic type. 5 of the Massachusetts genotypes are originated from recombination events between H120-like and other types of IBVs. Recombination resulted in the emergence of a novel serotype of IBV, although they are Massachusetts genotype by S1 gene analysis. In this study, 418 IBVs were isolated in samples from 1717 chicken flocks. Twenty-nine of the isolates were classified as the Massachusetts genotype. These 29 isolates, as well as two previously isolated Massachusetts genotype IBV strains, were studied further. Of the 31 strains, 24 were H120-like and two were M41-like isolates as determined by complete genomic sequence analysis, indicating that most of the IBV isolates were likely the reisolated vaccine virus. The remaining five IBV isolates, ck/CH/LHB/111172, ck/CH/LSD/111219, ck/CH/LHB/130598, ck/CH/LDL/110931, and ck/CH/LHB/130573, were shown to have originated from natural recombination events between an H120-like vaccine strain and other types of viruses. The virus cross-neutralization test found that the antigenicity of ck/CH/LHB/111172, ck/CH/LSD/111219, and ck/CH/LHB/130598 was similar to that of H120. Vaccination with the H120 vaccine offered complete protection against challenge with these isolates. However, isolates ck/CH/LDL/110931 and ck/CH/LHB/130573 were serotypically different from their parental viruses and from other serotypes in this study. Furthermore, vaccination with the H120 vaccine did not provide protection against challenge with these two isolates. The results of this study demonstrated that recombination is the mechanism that is responsible for the emergence of new serotype strains, and it has the ability to alter virus serotypes. Therefore, IBV surveillance of chicken flocks vaccinated with IBV live vaccines, as well as the consideration of new strategies to effectively control IBV infection using inactivated or/and genetically engineered vaccines, is of great importance.
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影响因子：
3.3
作者：
Liu X;Ma H;Xu Q;Sun N;Han Z;Sun C;Guo H;Shao Y;Kong X;Liu S
通讯作者：
Liu S
影响因子：
5
作者：
Cavanagh D;Davis PJ;Mockett AP
通讯作者：
Mockett AP
影响因子：
1.6
作者：
Callison, SA;Jackwood, MW;Hilt, DA
通讯作者：
Hilt, DA
影响因子：
5.5
作者：
Liu S;Zhang X;Gong L;Yan B;Li C;Han Z;Shao Y;Li H;Kong X
通讯作者：
Kong X
影响因子：
3.8
作者：
Liu, SW;Chen, JF;Liu, M
通讯作者：
Liu, M