Brain-wide mapping of c-Fos expression with fluorescence micro-optical sectioning tomography in a chronic sleep deprivation mouse model.
Brain-wide mapping of c-Fos expression with fluorescence micro-optical sectioning tomography in a chronic sleep deprivation mouse model.
复制标题
在慢性睡眠剥夺小鼠模型中，利用荧光显微光学断层扫描技术绘制全脑 c-Fos 表达图
DOI：
10.1016/j.ynstr.2022.100478
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发表时间：
2022-09
影响因子：
5
通讯作者：
Zhao, Yan
中科院分区：
文献类型：
--
作者：
Cai, Guohong;Lu, Yifan;Chen, Jing;Yang, Dingding;Yan, Ruixuan;Ren, Mudan;He, Shuixiang;Wu, Shengxi;Zhao, Yan
慢性睡眠剥夺（SD）对人类来说是一个常见的问题，它会导致许多有害的影响，包括抑郁、焦虑、中风、永久性认知缺陷、压力和其他生理疾病。获取全脑水平的相关神经活动信息对于系统地探讨SD相关的脑功能障碍机制至关重要。即时早期基因（IEG） Fos在小鼠大脑中的表达已被广泛用作神经科学领域的脑活动功能标志物。然而，以往的研究大多采用传统的研究方法或短期SD模型，只分析了几个特定脑区c-Fos的变化。在这里，我们通过荧光显微光学断层扫描（fMOST）技术和AAV-PHP应用c-Fos定位。eB综合分析慢性SD小鼠模型全脑累积激活状态。将小鼠移动到一个单独的充满水的保持区域，诱导慢性快速眼动（REM） SD模型。试验期为每天6 h。结果表明，SD 14 d后，小鼠在开阔场试验和升高加迷宫试验中表现出焦虑样行为，在悬尾试验和蔗糖偏好试验中表现出抑郁样行为。最多在230个脑区检测到c-Fos +细胞。与对照组相比，sd诱导的应激模型在多个脑区诱发了c-Fos的表达。特别是等皮质-大脑皮质板区，包括脾后、前扣带、颗粒岛、味觉和副耻骨，似乎是慢性REM SD后最敏感的区域。
Chronic sleep deprivation (SD) is a common problem for humans and can lead to many deleterious effects, including depression, anxiety, stroke, permanent cognitive deficits, stress, and other physiological diseases. It is vital to acquire information about the relevant neural activities at the whole-brain level to systematically explore the mechanisms of brain dysfunction related to SD. Expression of the immediate-early gene (IEG) Fos in the mouse brain has been widely used as a functional marker of brain activity in the field of neuroscience. However, most previous studies only analyzed the change of c-Fos in several specific brain regions using traditional research methods or in short-term SD model. Here, we applied c-Fos mapping through the fluorescence micro-optical sectioning tomography (fMOST) technique and AAV-PHP.eB to comprehensive analysis the state of cumulative activation across the whole brain in a mouse model of chronic SD. The chronic rapid eyes movement (REM) SD model was induced by moving mice to a separate holding area filled with water. The experimental period lasted for 6 h per day. The results showed that after 14 days of SD, the mice displayed anxiety-like behaviors in open field test and elevated plus maze test, and displayed depression-like behaviors in tail suspension test and the sucrose preference test. The c-Fos + cells were detected in a maximum of 230 brain regions. SD-induced stress model evoked c-Fos expression in several brain regions compared to the control group. In particular, the isocortex-cerebral cortex plate area, including the retrosplenial, anterior cingulate, agranular insular, gustatory, and parasubiculum, appear to be the most sensitive regions after chronic REM SD.
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影响因子：
3.2
作者：
Goldstein-Piekarski AN;Greer SM;Saletin JM;Harvey AG;Williams LM;Walker MP
通讯作者：
Walker MP
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影响因子：
10.6
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Costa, Rui M.
影响因子：
5.6
作者：
Knutson, Kristen L.;Van Cauter, Eve;Lauderdale, Diane S.
通讯作者：
Lauderdale, Diane S.
微光学断层扫描获得小鼠大脑的高分辨率图谱
影响因子：
56.9
作者：
Li, Anan;Gong, Hui;Luo, Qingming
通讯作者：
Luo, Qingming