CD68-positive tumour associated macrophages, PD-L1 expression, and EBV latent infection in a high HIV-prevalent South African cohort of Hodgkin lymphoma patients.
CD68-positive tumour associated macrophages, PD-L1 expression, and EBV latent infection in a high HIV-prevalent South African cohort of Hodgkin lymphoma patients.
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DOI：
10.1016/j.pathol.2020.11.004
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发表时间：
2021-08
期刊：
影响因子：
4.5
通讯作者：
Verburgh E
中科院分区：
文献类型：
--
作者：
Antel K;Chetty D;Oosthuizen J;Mohamed Z;Van der Vyver L;Verburgh E
在HIV阴性霍奇金淋巴瘤（HL）患者中，较高比例的CD 68阳性肿瘤相关巨噬细胞（TAM）与较差的结局相关，但在HIV阳性HL患者中是否如此尚不清楚。在这项研究中，我们研究了HL患者淋巴结标本中CD 68阳性TAM的数量和程序性细胞死亡配体1（PD-L1）的表达，以及与临床特征（HIV状态、疾病严重程度和生存率）和组织病理学特征（EBV潜伏阳性和HL亚型）相关的表达。我们对77例诊断为HL的患者的存档淋巴结标本进行了CD 68和PD-L1染色。染色分级为：CD 68低（≤25%）、CD 68高（>25%）、PD-L1低（≤50%）和PD-L1高（>50%）。使用双变量和多变量分析，表达水平与临床和组织病理学特征相关。通过总体生存期和无进展生存期分析生存期。纳入的77例患者中有34例（44%）为HIV阳性。在97%的HIV阳性HL患者和14%的HIV阴性HL患者中检测到EBV潜伏期。高CD 68评分与较低的中位血红蛋白水平相关（9.4 vs 11.4 g/dL; p=0.02），血小板数量（262 vs 424个细胞×109/L; p=0.01），淋巴细胞数量（0.99 vs 1.70个细胞×109/L，p=0.01）和晚期疾病趋势（国际预后评分≥4;风险比2.4;置信区间0.89-6.47; p=0.08）。HIV状态不影响CD 68或PD-L1表达。在EBV阳性的样本中发现了更高比例的CD 68阳性TAM。HIV阳性和EBV阴性与较差的生存率相关。CD 68和PD-L1表达不能预测生存率。CD 68高表达与EBV阳性相关，但与HIV阳性无关，也不能预测不良结局。PD-L1表达不受HIV状态或EBV阳性的影响，并预测不良结局。
A higher proportion of CD68-positive tumour associated macrophages (TAMs) has been associated with poorer outcomes in HIV-negative patients with Hodgkin lymphoma (HL), but whether this is true in HIV-positive patients with HL is not known. In this study, we investigated the number of CD68-positive TAMs and expression of programmed cell death-ligand 1 (PD-L1) in lymph node specimens from HL patients and correlated expression with clinical features (HIV status, disease severity and survival) and histopathological features (EBV latent positivity and subtype of HL). We stained archived lymph node specimens from 77 patients diagnosed with HL for CD68 and PD-L1. Stains were graded as: CD68 low (≤25%), CD68 high (>25%), PD-L1 low (≤50%), and PD-L1 high (>50%). Expression levels were correlated with the clinical and histopathological features using bivariate and multivariate analyses. Survival was analysed by overall and progression-free survival. Thirty-four of the 77 included patients (44%) were HIV-positive. EBV latency was detected in 97% of HIV-positive HL patients and in 14% of HIV-negative HL patients. A high CD68 score was associated with lower median haemoglobin levels (9.4 vs 11.4 g/dL; p=0.02), platelet numbers (262 vs 424 cells ×109/L; p=0.01), and lymphocyte numbers (0.99 vs 1.70 cells ×109/L, p=0.01) and a trend towards advanced disease (international prognostic score ≥4; hazard ratio 2.4; confidence interval 0.89–6.47; p=0.08). HIV status did not affect CD68 or PD-L1 expression. A higher proportion of CD68-positive TAMs was found in samples that were EBV-positive. HIV positivity and EBV negativity correlated with poorer survival. CD68 and PD-L1 expression were not predictive of survival. High CD68 expression was associated with EBV positivity but not HIV positivity and did not predict adverse outcomes. PD-L1 expression was unaffected by HIV status or EBV positivity and did predict adverse outcomes.
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影响因子：
2.2
作者：
Kiyasu, Junichi;Aoki, Ryosuke;Ohshima, Koichi
通讯作者：
Ohshima, Koichi
影响因子：
20.3
作者：
Green, Michael R.;Monti, Stefano;Shipp, Margaret A.
通讯作者：
Shipp, Margaret A.
影响因子：
6.4
作者：
Koulis, Athanasios;Trivedi, Pritesh;Naresh, Kikkeri N.
通讯作者：
Naresh, Kikkeri N.
DOI：
10.3324/haematol.2010.031542
发表时间：
2011-02-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
--
作者：
Kamper, Peter;Bendix, Knud;d'Amore, Francesco
通讯作者：
d'Amore, Francesco
影响因子：
2.6
作者：
LEVY, R;COLONNA, P;ANDRIEU, JM
通讯作者：
ANDRIEU, JM