Diet-induced obesity significantly increases the severity of posttraumatic arthritis in mice.
Diet-induced obesity significantly increases the severity of posttraumatic arthritis in mice.
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DOI：
10.1002/art.34533
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发表时间：
2012-10
影响因子：
--
通讯作者：
Guilak, Farshid
中科院分区：
文献类型：
--
作者：
Louer, Craig R.;Furman, Bridgette D.;Huebner, Janet L.;Kraus, Virginia B.;Olson, Steven A.;Guilak, Farshid
肥胖和关节损伤都是骨关节炎(OA)的主要危险因素，涉及关节生物力学和炎症环境的潜在变化。创伤后关节炎(PTA)是关节内骨折常见的长期并发症。肥胖与原发性骨性关节炎有关，并可能通过多种机制促进PTA的发展。本研究的目的是确定饮食诱导的肥胖是否影响小鼠PTA的严重程度，并检查关节退行性变与血清炎症细胞因子和脂肪因子水平之间的相互关系。C57BL/6小鼠从4周龄开始分别饲喂普通饲料(13%脂肪)和高脂饲料(60%脂肪)。16周时，每组各有一半接受闭合性左膝关节内骨折。骨折后8周，除进行骨组织学检查外，还对膝关节骨关节炎进行软骨和滑膜组织学检查。采用多重分析法检测血清细胞因子水平。与非骨折对照组相比，高脂饮食组小鼠骨折侧膝关节表现出明显的骨关节退行性变，而低脂饮食组小鼠骨折侧膝关节与非骨折对照组没有显著差异。高脂饮食增加血清白介素12p70、白介素6和角质形成细胞衍生趋化因子的浓度，同时降低脂联素浓度。在对照肢体中，全身脂联素水平与滑膜炎症呈负相关。饮食诱导的肥胖显著增加了关节内骨折后骨关节炎的严重程度。肥胖和关节损伤一起可以改变全身炎症细胞因子的水平，如IL-12p70。
Obesity and joint injury are both primary risk factors for osteoarthritis (OA) that involve potential alterations in the biomechanical and inflammatory environments of the joint. Post-traumatic arthritis (PTA) is a frequent long-term complication of intra-articular fractures. Obesity has been linked to primary OA and may potentially contribute to the development of PTA by a variety of mechanisms. The objectives of this study were to determine if diet-induced obesity influences the severity of PTA in mice and to examine interrelationships between joint degeneration and serum levels of inflammatory cytokines and adipokines in this response. C57BL/6 mice were fed either normal chow (13% fat) or a high-fat diet (60% fat) starting at 4 weeks of age. At 16 weeks, half of each group received closed intra-articular fracture of the left knee. At 8 weeks post-fracture, knee osteoarthritis was assessed by cartilage and synovium histology in addition to bone morphology. Serum cytokine concentrations were determined with multiplex assay. Fractured knee joints of mice on a high-fat diet showed significantly increased osteoarthritic degeneration compared to non-fractured contralateral controls, while fractured knee joints of low-fat mice did not demonstrate significant differences from non-fractured contralateral controls. High-fat diet increased serum concentrations of interleukin-12p70, interleukin-6, and keratinocyte-derived chemokine, while decreasing adiponectin concentrations. Systemic levels of adiponectin were inversely correlated with synovial inflammation in control limbs. Diet-induced obesity significantly increased the severity of osteoarthritis following intra-articular fracture. Obesity and joint injury together can alter systemic levels of inflammatory cytokines such as IL-12p70.
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影响因子：
4.6
作者：
Fain JN
通讯作者：
Fain JN
影响因子：
--
作者：
Griffin, Timothy M.;Huebner, Janet L.;Kraus, Virginia B.;Guilak, Farshid
通讯作者：
Guilak, Farshid
影响因子：
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作者：
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作者：
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作者：
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通讯作者：
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