The M1 form of tumor-associated macrophages in non-small cell lung cancer is positively associated with survival time.
The M1 form of tumor-associated macrophages in non-small cell lung cancer is positively associated with survival time.
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DOI：
10.1186/1471-2407-10-112
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发表时间：
2010-03-25
期刊：
影响因子：
3.8
通讯作者：
You Z
中科院分区：
文献类型：
--
作者：
Ma J;Liu L;Che G;Yu N;Dai F;You Z
肿瘤相关巨噬细胞（tumor associated macrophages, tam）在肿瘤的生长、进展和转移中起着重要作用。在非小细胞肺癌（NSCLC）中，tam的抗肿瘤或促肿瘤作用尚不确定。巨噬细胞极化为M1型（具有抗肿瘤功能）和M2型（具有促肿瘤功能）。本研究旨在确定NSCLC中M1和M2巨噬细胞密度是否与患者生存时间相关。本回顾性研究纳入平均生存期为1年的患者50例（短生存组）和平均生存期为5年的患者50例（长生存组）。使用石蜡包埋的NSCLC标本及其临床病理数据，包括长达8年的随访信息。采用盲法对CD68/HLA-DR （M1巨噬细胞标记物）和CD68/CD163 （M2巨噬细胞标记物）进行免疫组化双染色。采用计算机辅助显微镜检测肿瘤胰岛、间质、胰岛和间质中M1、M2巨噬细胞密度。使用社会科学统计软件包分析巨噬细胞密度与患者生存时间的相关性。在NSCLC中，约70%的tam为M2巨噬细胞，其余30%为M1巨噬细胞。在长生存组和短生存组之间，肿瘤胰岛、间质或胰岛和间质中的M2巨噬细胞密度（约为78 - 113 / mm2）无显著差异。长生存组肿瘤胰岛M1巨噬细胞密度（约70/mm2）和间质M1巨噬细胞密度（约34/mm2）显著高于短生存组肿瘤胰岛M1巨噬细胞密度（约7/mm2）和间质M1巨噬细胞密度（13/mm2） （P < 0.001和P < 0.05）。M2巨噬细胞密度与患者生存时间无相关性。单因素分析显示，肿瘤胰岛、间质或胰岛和间质中M1巨噬细胞密度与患者生存时间呈正相关（P < 0.01或0.001）。在多变量Cox比例风险分析中，肿瘤胰岛M1巨噬细胞密度是患者生存时间的独立预测因子。肿瘤胰岛M1巨噬细胞密度是非小细胞肺癌患者生存时间的独立预测因子。
Tumor-associated macrophages (TAMs) play an important role in growth, progression and metastasis of tumors. In non-small cell lung cancer (NSCLC), TAMs' anti-tumor or pro-tumor role is not determined. Macrophages are polarized into M1 (with anti-tumor function) and M2 (with pro-tumor function) forms. This study was conducted to determine whether the M1 and M2 macrophage densities in NSCLC are associated with patient's survival time. Fifty patients with an average of 1-year survival (short survival group) and 50 patients with an average of 5-year survival (long survival group) were included in this retrospective study. Paraffin-embedded NSCLC specimens and their clinicopathological data including up to 8-year follow-up information were used. Immunohistochemical double-staining of CD68/HLA-DR (markers for M1 macrophages) and CD68/CD163 (markers for M2 macrophages) was performed and evaluated in a blinded fashion. The M1 and M2 macrophage densities in the tumor islets, stroma, or islets and stroma were determined using computer-aided microscopy. Correlation of the macrophage densities and patient's survival time was analyzed using the Statistical Package for the Social Sciences. Approximately 70% of TAMs were M2 macrophages and the remaining 30% were M1 macrophages in NSCLC. The M2 macrophage densities (approximately 78 to 113 per mm2) in the tumor islets, stroma, or islets and stroma were not significantly different between the long survival and short survival groups. The M1 macrophage densities in the tumor islets (approximately 70/mm2) and stroma (approximately 34/mm2) of the long survival group were significantly higher than the M1 macrophage densities in the tumor islets (approximately 7/mm2) and stroma (13/mm2) of the short survival group (P < 0.001 and P < 0.05, respectively). The M2 macrophage densities were not associated with patient's survival time. The M1 macrophage densities in the tumor islets, stroma, or islets and stroma were positively associated with patient's survival time in a univariate analysis (P < 0.01 or 0.001). In a multivariate Cox proportional hazards analysis, the M1 macrophage density in the tumor islets was an independent predictor of patient's survival time. The M1 macrophage density in the tumor islets is an independent predictor of survival time in NSCLC patients.
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影响因子：
4.4
作者：
Baj-Krzyworzeka, Monika;Szatanek, Rafal;Zembala, Marek
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影响因子：
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影响因子：
2.8
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Moller, Bjarne Kuno
影响因子：
45.3
作者：
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通讯作者：
Bradding, P
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作者：
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