Temperature- and touch-sensitive neurons couple CNG and TRPV channel activities to control heat avoidance in Caenorhabditis elegans.
Temperature- and touch-sensitive neurons couple CNG and TRPV channel activities to control heat avoidance in Caenorhabditis elegans.
复制标题
DOI：
10.1371/journal.pone.0032360
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Baumeister R
中科院分区：
文献类型：
--
作者：
Liu S;Schulze E;Baumeister R
任何生物体都依赖于感知温度和躲避有害热量的能力。秀丽隐杆线虫（Caenorhabditis elegans）对超过~ 35°C的有害温度有反应，也能感知环境温度在15至25°C之间的变化。热趋向性的神经回路和分子机制已经被成功研究，而热回避行为的细节仍然难以捉摸。在这项工作中，我们利用遗传、细胞生物学和生理学的方法研究了热感觉的神经学和分子方面。我们在这里展示了热感觉神经元AFD，除了在动物可以茁壮成长的范围内感知温度外，还有助于感知有害的温度，从而导致反射式的逃逸反应。不同的中间神经元组分别参与传递热感觉和热趋向性。AFD的丧失部分由一对多树突、多模态神经元（FLP）的活动补偿，而激光消融这两种类型的神经元几乎完全消除了动物头部的热反应。第三对热感觉神经元PHC位于尾巴上。我们发现，热回避反应需要AFD中cGMP依赖性环核苷酸门控（CNG）通道的细胞自主功能，以及FLP和PHC感觉神经元中对热和辣椒素敏感的瞬时受体电位（TRPV）通道的细胞自主功能。我们的研究结果确定了由单个神经元介导的不同的热反应，但也表明平行的伤害感受器回路和分子可能被用作备用策略，以保证对潜在有害刺激的快速有效反应。
Any organism depends on its ability to sense temperature and avoid noxious heat. The nematode Caenorhabditis elegans responds to noxious temperatures exceeding ∼35°C and also senses changes in its environmental temperature in the range between 15 and 25°C. The neural circuits and molecular mechanisms involved in thermotaxis have been successfully studied, whereas details of the thermal avoidance behavior remain elusive. In this work, we investigate neurological and molecular aspects of thermonociception using genetic, cell biological and physiological approaches. We show here that the thermosensory neurons AFD, in addition to sensing temperature within the range within which the animals can thrive, also contribute to the sensation of noxious temperatures resulting in a reflex-like escape reaction. Distinct sets of interneurons are involved in transmitting thermonociception and thermotaxis, respectively. Loss of AFD is partially compensated by the activity of a pair of multidendritic, polymodal neurons, FLP, whereas laser ablation of both types of neurons abrogated the heat response in the head of the animals almost completely. A third pair of heat sensory neurons, PHC, is situated in the tail. We find that the thermal avoidance response requires the cell autonomous function of cGMP dependent Cyclic Nucleotide-Gated (CNG) channels in AFD, and the heat- and capsaicin-sensitive Transient Receptor Potential Vanilloid (TRPV) channels in the FLP and PHC sensory neurons. Our results identify distinct thermal responses mediated by a single neuron, but also show that parallel nociceptor circuits and molecules may be used as back-up strategies to guarantee fast and efficient responses to potentially detrimental stimuli.
登录
查看更多内容
影响因子：
9.8
作者：
Chang AJ;Chronis N;Karow DS;Marletta MA;Bargmann CI
通讯作者：
Bargmann CI
影响因子：
3.5
作者：
Albeg, Adi;Smith, Cody J.;Chatzigeorgiou, Marios;Feitelson, Dror G.;Hall, David H.;Schafer, William R.;Miller, David M., III;Treinin, Millet
通讯作者：
Treinin, Millet
影响因子：
16.2
作者：
Coburn, CM;Bargmann, CI
通讯作者：
Bargmann, CI
影响因子：
10.5
作者：
Baumeister, R;Liu, YX;Ruvkun, G
通讯作者：
Ruvkun, G
DOI：
10.1016/j.bbrc.2004.10.060
发表时间：
2004-12-10
影响因子：
3.1
作者：
Cho, SW;Choi, KY;Park, CS
通讯作者：
Park, CS