Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene.
Adaptation of Arabidopsis to nitrogen limitation involves induction of anthocyanin synthesis which is controlled by the NLA gene.
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DOI：
10.1093/jxb/ern148
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发表时间：
2008
影响因子：
6.9
通讯作者：
Rothstein SJ
中科院分区：
文献类型：
--
作者：
Peng M;Hudson D;Schofield A;Tsao R;Yang R;Gu H;Bi YM;Rothstein SJ
植物可以通过发展一系列的氮限制适应性反应来在有限的氮供应下生存。然而，拟南芥nla（氮限制适应）突变体不能产生这样的反应，并且不能适应氮限制。本研究利用nla突变体进一步研究了NLA对拟南芥适应氮限制的影响。生长限制N，nla突变体不能积累花青素，而是产生了N限制诱导的早衰表型。与此相反，当供应有限的N和有限的磷（Pi），nla突变体积累丰富的花青素，并没有显示出氮限制诱导的早衰表型。这些结果支持了这样的假设：拟南芥具有控制氮限制诱导的花色苷合成的特定途径，而nla突变破坏了这一途径。然而，nla突变不影响Pi限制诱导的花青素苷合成途径。因此，磷限制诱导nla突变体在氮限制下积累花青素，并允许该突变体适应氮限制。在氮限制下，nla突变体有一个显着下调表达的许多基因功能的花色素苷的合成，和增强的基因参与木质素的生产。相应地，nla突变体生长与限制N表现出显着较低的生产花青素（特别是花青素）和木质素含量的增加相比，野生型植物。这些数据表明，NLA控制拟南芥的适应性N限制通过引导苯丙烷类代谢通量诱导花青素的合成，这是重要的拟南芥适应N限制。
Plants can survive a limiting nitrogen (N) supply by developing a set of N limitation adaptive responses. However, the Arabidopsis nla (nitrogen limitation adaptation) mutant fails to produce such responses, and cannot adapt to N limitation. In this study, the nla mutant was utilized to understand further the effect of NLA on Arabidopsis adaptation to N limitation. Grown with limiting N, the nla mutant could not accumulate anthocyanins and instead produced an N limitation-induced early senescence phenotype. In contrast, when supplied with limiting N and limiting phosphorus (Pi), the nla mutants accumulated abundant anthocyanins and did not show the N limitation-induced early senescence phenotype. These results support the hypothesis that Arabidopsis has a specific pathway to control N limitation-induced anthocyanin synthesis, and the nla mutation disrupts this pathway. However, the nla mutation does not affect the Pi limitation-induced anthocyanin synthesis pathway. Therefore, Pi limitation induced the nla mutant to accumulate anthocyanins under N limitation and allowed this mutant to adapt to N limitation. Under N limitation, the nla mutant had a significantly down-regulated expression of many genes functioning in anthocyanin synthesis, and an enhanced expression of genes involved in lignin production. Correspondingly, the nla mutant grown with limiting N showed a significantly lower production of anthocyanins (particularly cyanidins) and an increase in lignin contents compared with wild-type plants. These data suggest that NLA controls Arabidopsis adaptability to N limitation by channelling the phenylpropanoid metabolic flux to the induced anthocyanin synthesis, which is important for Arabidopsis to adapt to N limitation.
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影响因子：
--
作者：
Gould KS
通讯作者：
Gould KS
影响因子：
7.4
作者：
Winkel-Shirley, B
通讯作者：
Winkel-Shirley, B
影响因子：
4.9
作者：
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通讯作者：
Maselaux-Daubresse, U
影响因子：
7.4
作者：
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通讯作者：
FOYER, C
影响因子：
4.2
作者：
Ding, L;Wang, KJ;Li, YH
通讯作者：
Li, YH