In vitro and in vivo assessment of the effect of antiprotozoal compounds isolated from Psoralea corylifolia against Ichthyophthirius multifiliis in fish.
In vitro and in vivo assessment of the effect of antiprotozoal compounds isolated from Psoralea corylifolia against Ichthyophthirius multifiliis in fish.
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从补骨脂中分离的抗原虫化合物对鱼类多子鱼霉作用的体外和体内评估
DOI：
10.1016/j.ijpddr.2015.04.001
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发表时间：
2015-08
影响因子：
4
通讯作者：
Wang, Gaoxue
中科院分区：
文献类型：
--
作者：
Song, Kaige;Ling, Fei;Huang, Aiguo;Dong, Wenjing;Liu, Guanglu;Jiang, Chao;Zhang, Qizhong;Wang, Gaoxue
从紫堇中分离并鉴定了两种抗原虫化合物。对异补骨脂素和补骨脂素的体外抗原虫效果进行了评价。在原位条件下，补骨脂素对多丝硬蜱的营养体有一定的破坏作用。探讨了其对多丝硬蜱的作用机理。多丝小瓜虫是一种外部鱼类寄生虫，经常对水产养殖业造成重大经济损失。由于孔雀石绿色的使用被禁止，寻找替代物质来控制I. multifiliis感染变得更加严格。本研究以抗脑出血的成囊绒毛为基础，采用生物活性测定法，对从补骨脂甲醇提取物中分离得到的两种活性成分异补骨脂素和补骨脂定进行了体内外抗脑出血活性评价。在体外实验中，补骨脂素的抗原虫效果明显优于异补骨脂素。在0.8mg/L以上浓度下，暴露4 h即可杀死所有的刺蛾，在0.9mg/L和1.2mg/L被囊刺蛾暴露6 h后，即可终止多丝硬蜱的繁殖。体内试验表明，2.5mg/L的peptidin暴露于感染鱼5 h，显著减少了从绒毛释放的theronts的数量。此外，我们观察到处理后从感染鱼中采集的部分原胞体在Annexin V-EGFP/碘化丙啶染色后出现了特征性的细胞凋亡形态学变化，这表明补骨脂素原位抗多丝硬蜱滋养体的可能机制。基于这些结果，palcidin可作为潜在的先导化合物用于开发抗多丝硬蜱的商业药物。
Two antiprotozoal compounds from P. corylifolia were isolated and identified. In vitro antiprotozoal efficacies of isopsoralen and psoralidin were evaluated. Psoralidin had a detrimental effect on I. multifiliis trophont in situ. The action mechanism against I. multifiliis trophont in situ was investigated. Ichthyophthirius multifiliis, an external fish parasite, often causes significant economic damage to the aquaculture industry. Since the use of malachite green was banned, the search of alternative substance to control I. multifiliis infections becomes stringent. In present study, in vitro and in vivo anti-ich efficacies of isopsoralen and psoralidin, two active compounds isolated from methanol extract of Psoralea corylifolia by bioassay-guided fractionation based on the efficacy of anti-ich encysted tomonts, were evaluated. In vitro antiprotozoal efficacy of psoralidin is much better than that of isopsoralen. Psoralidin can kill all theronts at concentrations of 0.8 mg/L or more during 4 h exposure; and terminate reproduction of I. multifiliis post 6 h exposure of protomonts to 0.9 mg/L and encysted tomonts to 1.2 mg/L. In vivo trials showed that 5 h exposure of infected fish to 2.5 mg/L of psoralidin significantly reduced the number of theronts released from tomonts. Furthermore, we observed that a part of protomonts, collected from infected fish post treatment, presented characteristic morphological changes of apoptosis after staining with Annexin V-EGFP/propidium iodide, indicating the possible mechanism of psoralidin against I. multifiliis trophont in situ. On the basis of these results, psoralidin can be used as a potential lead compound for the development of commercial drug against I. multifiliis.
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影响因子：
4.1
作者：
Xiao, Guodong;Li, Guowen;Wang, Zhengtao
通讯作者：
Wang, Zhengtao
影响因子：
2.6
作者：
Ling, Fei;Wang, Jian-Guo;Gong, Xiao-Ning
通讯作者：
Gong, Xiao-Ning
影响因子：
4.1
作者：
Picon-Camacho, Sara M.;Leclercq, Eric;Shinn, Andrew P.
通讯作者：
Shinn, Andrew P.
影响因子：
6.7
作者：
Lee, Sung-Jin;Nam, Kung-Woo;Mar, Woongchon
通讯作者：
Mar, Woongchon
影响因子：
2.5
作者：
CLAYTON, GM;PRICE, DJ
通讯作者：
PRICE, DJ