A Novel Method for Evaluating Postoperative Adhesions in Rats.
A Novel Method for Evaluating Postoperative Adhesions in Rats.
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DOI：
10.1080/08941939.2016.1229367
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发表时间：
2017-04
期刊：
影响因子：
--
通讯作者：
Hartvigsen J
中科院分区：
文献类型：
--
作者：
Bove GM;Chapelle SL;Boyle E;Mokler DJ;Hartvigsen J
术后粘连仍然是腹盆手术的不良反应和常见症状。术后粘连的动物模型通常在整个腹腔内产生异质性粘连，并且不容易量化。在这里，我们提出了一种新的术后粘连评估方法，并报告其可靠性和测量误差。采用雌性大鼠建立盲肠部分侧壁附着磨损模型。恢复1、2、4或7天后，对大鼠实施安乐死，并系统评价其腹盆腔的术后粘连。通过手术显微镜记录尸检。4名评分员接受培训，在查看记录时使用投票记录粘连的关键因素。他们的评级进行了比较的测量误差和可靠性（分别使用Bland-Altman图和组内相关系数）和区分实验组差异的能力。分析数据的子集以确定实际效用。该评级系统被证明具有较低的测量误差和较高的评分员之间的所有参数测量的可靠性。在实际应用中，该系统能够以预期的方式区分群体。我们已经开发并验证了术后粘连的评级系统，并表明它可以检测组间差异。该方法可用于定量啮齿动物模型中的术后粘连。
Postoperative adhesions remain an undesirable and commonly symptomatic side effect of abdominopelvic surgeries. Animal models of postoperative adhesions typically yield heterogeneous adhesions throughout the abdominal cavity and are not easily quantified. Here we present a novel method of postoperative adhesion assessment and report its reliability and measurement error. A model of cecal abrasion with partial sidewall attachment was performed on female rats. After 1, 2, 4, or 7 days of recovery, the rats were euthanized and their abdominopelvic cavities were systematically evaluated for postoperative adhesions. The necropsy was recorded through the surgical microscope. Four raters were trained to use a ballot to capture key factors of the adhesions as they viewed the recordings. Their ratings were compared for measurement error and reliability (using Bland-Altman plots and intraclass correlation coefficients, respectively) and for the ability to discriminate differences in experimental groups. A subset of the data was analyzed to determine practical utility. The rating system was shown to have low measurement error and high inter-rater reliability for all parameters measured. Applied practically, the system was able to discriminate groups in a manner that was expected. We have developed and validated a rating system for postoperative adhesions and shown that it can detect group differences. This method can be used to quantify postoperative adhesions in rodent models.
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影响因子：
6.7
作者：
Fiedler, EP;KanbourShakir, A;Guido, R
通讯作者：
Guido, R
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2.2
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影响因子：
6.7
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通讯作者：
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