An Alternative Strategy for Trypanosome Survival in the Mammalian Bloodstream Revealed through Genome and Transcriptome Analysis of the Ubiquitous Bovine Parasite Trypanosoma (Megatrypanum) theileri.
An Alternative Strategy for Trypanosome Survival in the Mammalian Bloodstream Revealed through Genome and Transcriptome Analysis of the Ubiquitous Bovine Parasite Trypanosoma (Megatrypanum) theileri.
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DOI：
10.1093/gbe/evx152
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发表时间：
2017-08-01
影响因子：
3.3
通讯作者：
Carrington M
中科院分区：
文献类型：
--
作者：
Kelly S;Ivens A;Mott GA;O'Neill E;Emms D;Macleod O;Voorheis P;Tyler K;Clark M;Matthews J;Matthews K;Carrington M
有数百种锥虫物种生活在它们的脊椎动物宿主的血液和组织空间中。其中绝大多数没有在少数非洲锥虫物种中进化出的华丽的抗原变异系统，但在面对脊椎动物的适应性免疫系统时仍然可以保持长期感染。泰勒锥虫就是一个典型的例子，牛和其他牛科动物的宿主范围有限，虽然没有对感染对农业生产力的影响进行系统调查，但仅偶尔报告会导致专利疾病。在这里，一个详细的基因组序列和转录组分析血液中的基因表达泰勒氏锥虫已经完成。对基因组序列和表达的分析表明，T.theileri具有典型的动质体基因组结构，并能够预测它能够进行减数分裂交换，通过RNA干扰实现基因沉默，以及潜在的密度依赖的生长控制。特别是，转录组分析使两种截然不同的锥虫细胞表面--布鲁氏锥虫和泰勒锥虫--进行了比较，这两种锥虫各自进化为能够维持牛的长期细胞外感染。泰勒氏锥虫的细胞表面可以被模拟为含有由四个新的大而不同的基因家族和一个主要的表面蛋白酶基因家族的成员编码的蛋白质的混合物。这种表面成分不同于非洲锥虫表面统一的变异表面糖蛋白外壳，为锥虫物种在脊椎动物宿主的细胞外环境中增殖以避免适应性免疫反应所使用的第二种机制提供了洞察。
There are hundreds of Trypanosoma species that live in the blood and tissue spaces of their vertebrate hosts. The vast majority of these do not have the ornate system of antigenic variation that has evolved in the small number of African trypanosome species, but can still maintain long-term infections in the face of the vertebrate adaptive immune system. Trypanosoma theileri is a typical example, has a restricted host range of cattle and other Bovinae, and is only occasionally reported to cause patent disease although no systematic survey of the effect of infection on agricultural productivity has been performed. Here, a detailed genome sequence and a transcriptome analysis of gene expression in bloodstream form T. theileri have been performed. Analysis of the genome sequence and expression showed that T. theileri has a typical kinetoplastid genome structure and allowed a prediction that it is capable of meiotic exchange, gene silencing via RNA interference and, potentially, density-dependent growth control. In particular, the transcriptome analysis has allowed a comparison of two distinct trypanosome cell surfaces, T. brucei and T. theileri, that have each evolved to enable the maintenance of a long-term extracellular infection in cattle. The T. theileri cell surface can be modeled to contain a mixture of proteins encoded by four novel large and divergent gene families and by members of a major surface protease gene family. This surface composition is distinct from the uniform variant surface glycoprotein coat on African trypanosomes providing an insight into a second mechanism used by trypanosome species that proliferate in an extracellular milieu in vertebrate hosts to avoid the adaptive immune response.
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影响因子：
3.3
作者：
Cavalier-Smith, Thomas
通讯作者：
Cavalier-Smith, Thomas
影响因子：
64.5
作者：
Akiyoshi B;Gull K
通讯作者：
Gull K
影响因子：
2.2
作者：
DOHERTY, ML;WINDLE, H;MURRAY, M
通讯作者：
MURRAY, M
影响因子：
3.8
作者：
dos Santos SL;Freitas LM;Lobo FP;Rodrigues-Luiz GF;Mendes TA;Oliveira AC;Andrade LO;Chiari E;Gazzinelli RT;Teixeira SM;Fujiwara RT;Bartholomeu DC
通讯作者：
Bartholomeu DC
DOI：
10.1083/jcb.201506114
发表时间：
2015-10-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Domingo-Sananes MR;Szöőr B;Ferguson MA;Urbaniak MD;Matthews KR
通讯作者：
Matthews KR