Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by Inter-Simpe Sequence Repeat (ISSR) Markers.
Genetic diversity of the endemic and medicinally important plant Rheum officinale as revealed by Inter-Simpe Sequence Repeat (ISSR) Markers.
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DOI：
10.3390/ijms13033900
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发表时间：
2012
影响因子：
5.6
通讯作者：
Ren Y
中科院分区：
文献类型：
--
作者：
Wang XM;Hou XQ;Zhang YQ;Yang R;Feng SF;Li Y;Ren Y
大黄，一种重要但濒临灭绝的药用植物，是中国特有的。采用简单序列重复序列间（ISSR）标记对12个杜鹃居群的遗传多样性和分化进行了研究。officinale。筛选出13个引物，共扩增出189条清晰可辨的条带，平均每个引物扩增出14.54条。POPGENE分析结果表明，种群水平的遗传多样性较低，种水平的遗传多样性较高（H = 0.1008，I = 0.1505，PPB = 28. 95% vs. H = 0.3341，I = 0.5000，PPB = 95.24%）。分子方差分析（AMOVA）表明，居群间的遗传变异占74.38%，与居群间有限的基因流（Nm = 0.2766）相一致。Mantel检验显示，遗传距离与地理距离之间存在显著相关性（r = 0.5381，P = 0.002），表明地理隔离在形成当前群体遗传结构中的作用。贝叶斯聚类分析和UPGMA聚类分析的结果基本一致。我们的研究结果意味着，保护工作的目标应该是保护所有现存的种群这一濒危物种，并在这项研究中提出了种植。
Rheum officinale Baill., an important but endangered medicinal herb, is endemic to China. Inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 12 populations of R. officinale. Thirteen selected primers yielded 189 bright and discernible bands, with an average of 14.54 per primer. The genetic diversity was low at the population level, but pretty high at the species level (H = 0.1008, I = 0.1505, PPB = 28.95% vs. H = 0.3341, I = 0.5000, PPB = 95.24%, respectively) by POPGENE analysis. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly among populations (74.38%), in line with the limited gene flow (Nm = 0.2766) among populations. Mantel test revealed a significant correlation between genetic and geographic distances (r = 0.5381, P = 0.002), indicating the role of geographic isolation in shaping the present population genetic structure. Both Bayesian analysis and UPGMA cluster analysis demonstrated the similar results. Our results imply that the conservation efforts should aim to preserve all the extant populations of this endangered species, and cultivation is proposed in this study.
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