Novel beta-lactamase-random peptide fusion libraries for phage display selection of cancer cell-targeting agents suitable for enzyme prodrug therapy.
Novel beta-lactamase-random peptide fusion libraries for phage display selection of cancer cell-targeting agents suitable for enzyme prodrug therapy.
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DOI：
10.3109/10611860903244181
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发表时间：
2010-02
影响因子：
4.5
通讯作者：
Krag DN
中科院分区：
文献类型：
--
作者：
Shukla GS;Krag DN
Novel phage-displayed random linear dodecapeptide (X12) and cysteine-constrained decapeptide (CX10C) libraries constructed in fusion to the amino-terminus of P99 β-lactamase molecules were used for identifying β-lactamase-linked cancer cell-specific ligands. The size and quality of both libraries were comparable to the standards of other reported phage display systems. Using the single-round panning method based on phage DNA recovery, we identified severalβ-lactamase fusion peptides that specifically bind to live human breast cancer MDA-MB-361 cells. The β-lactamase fusion to the peptides helped in conducting the enzyme activity-based clone normalization and cell-binding screening in a very time- and cost-efficient manner. The methods were suitable for 96-well readout as well as microscopic imaging. The success of the biopanning was indicated by the presence of ~40% cancer cell-specific clones among recovered phages. One of the binding clones appeared multiple times. The cancer cell-binding fusion peptides also shared several significant motifs. This opens a new way of preparing and selecting phage display libraries. The cancer cell-specific β-lactamase-linked affinity reagents selected from these libraries can be used for any application that requires a reporter for tracking the ligand molecules. Furthermore, these affinity reagents have also a potential for their direct use in the targeted enzyme prodrug therapy of cancer.
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影响因子：
19
作者：
Naik, RR;Jones, SE;Stone, MO
通讯作者：
Stone, MO
影响因子：
7.8
作者：
Irving MB;Pan O;Scott JK
通讯作者：
Scott JK
影响因子：
15
作者：
Gao, WZ;Xing, BG;Rao, JH
通讯作者：
Rao, JH
影响因子：
4.9
作者：
OCALLAGHAN, CH;SHINGLER, AH;MORRIS, A
通讯作者：
MORRIS, A
影响因子：
3
作者：
Martin, J;Stribbling, SM;Springer, CJ
通讯作者：
Springer, CJ