Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.
Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes.
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DOI：
10.1093/nar/17.12.4713
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发表时间：
1989-06-26
影响因子：
14.9
通讯作者：
Blinov VM
中科院分区：
文献类型：
--
作者：
Gorbalenya AE;Koonin EV;Donchenko AP;Blinov VM
在对含有嘌呤NTP结合基序的蛋白质序列进行系统分析的过程中，一个新的超家族被描绘出来，其中包括25个已建立的或推定的解旋酶，包括大肠杆菌、酵母、昆虫、哺乳动物、痘病毒和疱疹病毒、酵母线粒体质粒和三组正链RNA病毒。这些蛋白含有7个不同的高度保守的片段，其中两个对应于“A”和“B”位点的NTP结合基序。新的超家族的典型特征是A位点的简化共识GxGKS/T，而不是经典的G/AxxxxGKS/T。二级结构预测表明，每个保守的片段可能构成一个单独的结构单元集中在一个β-转角。所有先前表征的突变损害酵母解旋酶RAD 3在DNA修复中的功能，映射到保守片段之一。一个新的超家族和另一个最近描述的蛋白质超家族，包括一个不同的原核生物，真核生物和病毒（推定）解旋酶的保守氨基酸残基的共识模式之间的相似性程度被揭示。
In the course of systematic analysis of protein sequences containing the purine NTP-binding motif, a new superfamily was delineated which included 25 established or putative helicases of Escherichia coli, yeast, insects, mammals, pox- and herpesviruses, a yeast mitochondrial plasmid and three groups of positive strand RNA viruses. These proteins contained 7 distinct highly conserved segments two of which corresponded to the "A" and "B" sites of the NTP-binding motif. Typical of the new superfamily was an abridged consensus for the "A" site, GxGKS/T, instead of the classical G/AxxxxGKS/T. Secondary structure predictions indicated that each of the conserved segments might constitute a separate structural unit centering at a beta-turn. All previously characterized mutations impairing the function of the yeast helicase RAD3 in DNA repair mapped to one of the conserved segments. A degree of similarity was revealed between the consensus pattern of conserved amino acid residues derived for the new superfamily and that of another recently described protein superfamily including a different set of prokaryotic, eukaryotic and viral (putative) helicases.
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影响因子：
3.7
作者：
CASTLE, E;NOWAK, T;WENGLER, G
通讯作者：
WENGLER, G
影响因子：
64.5
作者：
HAY, B;JAN, LY;JAN, YN
通讯作者：
JAN, YN
影响因子：
14.9
作者：
FORSTER, RLS;BEVAN, MW;GARDNER, RC
通讯作者：
GARDNER, RC
DOI：
10.1073/pnas.83.4.907
发表时间：
1986-02-01
影响因子：
11.1
作者：
FRY, DC;KUBY, SA;MILDVAN, AS
通讯作者：
MILDVAN, AS
影响因子：
3.7
作者：
COLLETT, MS;LARSON, R;PURCHIO, AF
通讯作者：
PURCHIO, AF