Prevalence, antibiotic resistance and genetic diversity of Listeria monocytogenes isolated from retail ready-to-eat foods in China
Prevalence, antibiotic resistance and genetic diversity of Listeria monocytogenes isolated from retail ready-to-eat foods in China
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中国零售即食食品中单核细胞增生李斯特氏菌的流行率、抗生素耐药性和遗传多样性
DOI：
10.1016/j.foodcont.2014.07.028
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发表时间：
2015
期刊：
影响因子：
6
通讯作者：
Yan Zean
中科院分区：
文献类型：
--
作者：
Wu Shi;Wu Qingping;Zhang Jumei;Chen Moutong;Yan Zean
单核细胞增生李斯特菌是一种主要的食源性致病菌，感染后死亡率高。本研究旨在调查l的患病率。中国零售即食（RTE）食品中的单核细胞原性分离株及其特征。通过抗生素耐药性、血清分型、ERIC-PCR和REP-PCR分型分析单核细胞增生。2012年9月至2014年1月，共获得零售RTE食品364种。采用定性和定量方法，25份样品（6.87%）l阳性。monocytogenes。分离菌株的鉴定。经PCR证实为单核细胞增生。80株菌株均对青霉素和甲洛西林敏感，其中克林霉素耐药率最高（51.25%），其次为头孢菌素（23.75%）和氨苄西林（12.5%）。27株菌株对所有14种试验抗生素均敏感；17株对2种以上抗生素耐药，其中6株对10种以上抗生素耐多药。单核细胞增生分离株属于血清型1/2a （3a）、4b （4d、4e）、1/2b （3b、7）和1/2c （3c）。29 l。通过血清分型筛选单核细胞生成分离株。在相对相似系数为0.80的情况下，通过ERIC-PCR和REP-PCR将29株分离菌株和5株参考菌株分别分为2簇3单株和4簇1单株。我们的研究反映了l的潜在风险。中国的单核细胞增生感染。我们还提供了一个全面的监测，其发生率的RTE食品的l。单核细胞增生，并确保更准确地治疗人类李斯特菌病有效的抗生素。
Listeria monocytogenes is a major foodborne pathogen that is well known as high mortality rate upon infected. This study aimed to investigate the prevalence ofL. monocytogenesisolates from retail ready-to-eat (RTE) foods in China and characterize the isolates ofL. monocytogenesby antibiotic resistance, serotyping, ERIC-PCR and REP-PCR subtyping analyses. From September 2012 to January 2014, a total of 364 retail RTE foods were obtained. Using the qualitative and quantitative methods, 25 samples (6.87%) were positive forL. monocytogenes. The identity of isolates ofL. monocytogeneswas confirmed by PCR. All 80 isolates in this survey were sensitive to penicillin and mezlocillin, the highest resistance is clindamycin (51.25%), followed by cephalothin (23.75%) and ampicillin (12.5%). Twenty-seven isolates were susceptible to all 14 tested antibiotics; seventeen isolates were resistant to more than two antibiotics, including six multiresistent strains resist to more than 10 antibiotics.L. monocytogenesisolates belonged to serovar types 1/2a (3a), 4b (4d, 4e), 1/2b (3b, 7) and 1/2c (3c). 29L. monocytogenesisolates were selected by serotyping. At the relative similarity coefficient of 0.80, it grouped 29 isolates and 5 reference strains into 2 clusters and 3 singletons, 4 clusters and 1 singleton by ERIC-PCR and REP-PCR, respectively. Our study reflects the potential risk ofL. monocytogenesinfection in China. We also provide a comprehensive surveillance on its incidence on the RTE foods ofL. monocytogenesand ensure more accurate treatment of human listeriosis with effective antibiotics.
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DOI：
10.1002/9780470752616.ch25
发表时间：
2008-02
期刊：
--
影响因子：
--
作者：
S. Kathariou
通讯作者：
S. Kathariou
影响因子：
2.8
作者：
BUCHRIESER, C;BROSCH, R;ROCOURT, J
通讯作者：
ROCOURT, J
影响因子：
5.4
作者：
Yifan Zhang;Emily Yeh;G. Hall;J. Cripe;A. Bhagwat;J. Meng
通讯作者：
Yifan Zhang;Emily Yeh;G. Hall;J. Cripe;A. Bhagwat;J. Meng
影响因子：
2.2
作者：
Li Ying-yue
通讯作者：
Li Ying-yue
影响因子：
9.4
作者：
Barbara Jeršek;P. Gilot;M. Gubina;N. Klun;J. Mehle;E. Tcherneva;N. Rijpens;L. Herman
通讯作者：
Barbara Jeršek;P. Gilot;M. Gubina;N. Klun;J. Mehle;E. Tcherneva;N. Rijpens;L. Herman