MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus.
MIR2111-5 locus and shoot-accumulated mature miR2111 systemically enhance nodulation depending on HAR1 in Lotus japonicus.
复制标题
DOI：
10.1038/s41467-020-19037-9
复制
发表时间：
2020-10-15
影响因子：
16.6
通讯作者：
Kawaguchi M
中科院分区：
文献类型：
--
作者：
Okuma N;Soyano T;Suzaki T;Kawaguchi M
豆科植物利用枝条介导的信号系统与根瘤中的固氮菌保持互惠关系。在莲花中，针对根中的结瘤抑制因子太多LOVE的microRNA miR2111被提出用来解释从地上部分控制结瘤的机制。然而，积累新梢的miR2111s在系统调控结瘤中的作用尚不清楚。在这里，我们发现日本血吸虫有7个miR2111基因座，包括通过RNA-seq定位的那些。在miR2111基因座中，MIR2111-5在叶片中的表达最高，在根瘤菌侵染后，MIR2111-5的表达受到抑制，这取决于HAR1受体的作用。MIR2111-5基因敲除突变体的根瘤数和miR2111水平显著降低。此外，使用转化子进行的嫁接实验表明，miR2111水平改变的接穗以剂量依赖的方式影响砧木中的根瘤数量。因此，通过表达MIR2111-5在叶片中积累miR2111对于依赖HAR1的系统优化根瘤数是必要的。在豆科植物中，枝条信号调节根瘤菌的共生，以平衡氮气固定的好处和根瘤生产的能源成本。在这里，Okuma等人。结果表明，莲花miR2111是从MIR2111-5基因座的叶片中产生的，是一种可嫁接传递的信号，可以促进根的结瘤。
Legumes utilize a shoot-mediated signaling system to maintain a mutualistic relationship with nitrogen-fixing bacteria in root nodules. In Lotus japonicus, shoot-to-root transfer of microRNA miR2111 that targets TOO MUCH LOVE, a nodulation suppressor in roots, has been proposed to explain the mechanism underlying nodulation control from shoots. However, the role of shoot-accumulating miR2111s for the systemic regulation of nodulation was not clearly shown. Here, we find L. japonicus has seven miR2111 loci, including those mapped through RNA-seq. MIR2111-5 expression in leaves is the highest among miR2111 loci and repressed after rhizobial infection depending on a shoot-acting HYPERNODULATION ABERRANT ROOT FORMATION1 (HAR1) receptor. MIR2111-5 knockout mutants show significantly decreased nodule numbers and miR2111 levels. Furthermore, grafting experiments using transformants demonstrate scions with altered miR2111 levels influence nodule numbers in rootstocks in a dose-dependent manner. Therefore, miR2111 accumulation in leaves through MIR2111-5 expression is required for HAR1-dependent systemic optimization of nodule number. In legumes, shoot-derived signals regulate rhizobial symbiosis to balance the benefits of N2 fixation with the energy costs of nodule production. Here Okuma et al. show that Lotus miR2111 is produced in the leaves from the MIR2111-5 locus and is a graft-transmissable signal that enhances nodulation in the roots.
登录
查看更多内容
影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
64.8
作者：
Krusell, L;Madsen, LH;Stougaard, J
通讯作者：
Stougaard, J
影响因子：
9.2
作者：
Gautrat, Pierre;Laffont, Carole;Frugier, Florian
通讯作者：
Frugier, Florian
影响因子：
6.9
作者：
Gautrat, Pierre;Mortier, Virginie;Goormachtig, Sofie
通讯作者：
Goormachtig, Sofie
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T