Effect of maternal protein restriction on lipid metabolism in Meishan piglets at weaning
Effect of maternal protein restriction on lipid metabolism in Meishan piglets at weaning
复制标题
母源蛋白质限制对断奶时梅山仔猪脂质代谢的影响
DOI：
10.1016/j.livsci.2010.08.013
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发表时间：
2011-04
影响因子：
1.8
通讯作者：
Xiaojing Yang
中科院分区：
文献类型：
--
作者：
Xiaowei Zhang;Jing Liang;Ruqian Zhao;Steffen Maak;Xiaojing Yang
本研究以梅山猪为模型，探讨母体蛋白限制对断奶仔猪脂代谢的影响及其机制。16头梅山母猪随机分为对照组和母体蛋白质限制组（MPR）。治疗组在怀孕期间喂食含6%蛋白质的低蛋白饮食，然后在哺乳期间喂食含7%蛋白质的低蛋白饮食，而对照组在怀孕和哺乳期间分别喂食12%和14%蛋白质。在断奶时采集仔猪的血液和皮下脂肪。结果表明，与对照组相比，MPR组仔猪的体重和背膘厚显著降低（P<0.05）。MPR组皮下脂肪组织中瘦素mRNA表达显著下调，血清瘦素浓度无明显变化，但瘦素含量呈下降趋势（P=0.10）。MPR组皮下脂肪中脂肪分解脂肪酶活性（包括激素敏感脂肪酶HSL和脂肪组织甘油三酯脂肪酶ATGL）显著升高（P<0.05）。MPR组脂肪酸合成酶（FAS）mRNA表达较对照组明显降低（P<0.05），而CCTTA增强子结合蛋白（C/EBP-β）和过氧化物酶体增殖物激活受体γ（PPAR-γ）mRNA表达无明显变化。MPR组总Perilipin蛋白水平无明显变化，但Perilipin mRNA表达明显降低，Perilipin蛋白磷酸化水平呈上升趋势（P=0.09）。本研究表明，母体蛋白限制组仔猪断奶时较低的脂质沉积可能是通过增加皮下脂肪的脂解作用介导的，其表现为脂解脂肪酶活性增加和围脂蛋白磷酸化水平升高。这些改变可能会影响以后生活的脂质代谢。
The present study aimed to determine the effects of maternal protein restriction on lipid metabolism of piglets at weaning and the associated underlying mechanism employing Meishan pigs as model. Sixteen Meishan sows were assigned to the control group and the maternal protein restriction group (MPR). The treated group was fed a low-protein diet containing 6% protein during pregnancy followed by 7% protein during lactation, whereas the control group received 12% and 14% protein during pregnancy and lactation, respectively. Blood and subcutaneous fat of piglets were sampled at weaning. The results showed that in MPR piglets' body weight and backfat thickness were significantly decreased compared to control piglets (P<0.05). Leptin mRNA expression was significantly down-regulated and the leptin content showed a decreased tendency (P=0.10) in subcutaneous fat of MPR group though the serum leptin concentration was unchanged. The lipolytic lipase activity (including Hormone sensitive lipase, HSL and adipose tissue triglyceride lipase, ATGL) in subcutaneous fat was significantly increased in MPR group (P<0.05). The fatty acid synthase (FAS) mRNA expression decreased significantly (P<0.05) although CCTTA enhancer-binding protein (C/EBP-β) and peroxisome proliferators-activated receptor γ (PPAR-γ) mRNA expression showed no obviously changes in MPR group compared with the control. Furthermore, though no alteration was detected for total perilipin protein level, the mRNA expression of perilipin was significantly decreased and the phosphorylation level of perilipin protein demonstrated an increased tendency (P=0.09) in the MPR group. The present study indicates that the lower lipid deposition in piglets of maternal protein restriction group at weaning may be mediated by the increased lipolysis in the subcutaneous fat demonstrated by increased lipolytic lipase activity and higher perilipin phosphorylation level. These alterations may influence the lipid metabolism of later life.
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影响因子：
3.3
作者：
T. Ramsay
通讯作者：
T. Ramsay
DOI：
10.1093/ajcn/70.5.811
发表时间：
1999-11
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
A. Ravelli;J. Meulen;C. Osmond;D. Barker;O. Bleker
通讯作者：
A. Ravelli;J. Meulen;C. Osmond;D. Barker;O. Bleker
影响因子：
6.9
作者：
Bol, Vanesa V.;Reusens, Brigitte M.;Remacle, Claude A.
通讯作者：
Remacle, Claude A.
影响因子：
17.3
作者：
C. Stocker;M. Cawthorne
通讯作者：
C. Stocker;M. Cawthorne
DOI：
10.1152/ajpregu.90497.2008
发表时间：
2009-08-01
影响因子：
2.8
作者：
Bol, V. V.;Delattre, A-I.;Remacle, C.
通讯作者：
Remacle, C.