Silver nanoparticles incite size- and dose-dependent developmental phenotypes and nanotoxicity in zebrafish embryos.
Silver nanoparticles incite size- and dose-dependent developmental phenotypes and nanotoxicity in zebrafish embryos.
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DOI：
10.1021/tx400228p
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发表时间：
2013-10-21
影响因子：
4.1
通讯作者：
Xu XH
中科院分区：
文献类型：
--
作者：
Browning LM;Lee KJ;Nallathamby PD;Xu XH
纳米材料具有独特的物理化学性质，从先进技术到前沿医学，有着广泛的应用前景。然而，它们对生物体的影响在很大程度上仍然未知。在这里，我们报告了纯化的银纳米颗粒（Ag NPs，97 ± 13 nm）以剂量依赖的方式激发特定发育阶段的胚胎表型和纳米毒性，在给定阶段的胚胎急性暴露于NPs（0-24 pM）仅2小时。NPs对卵裂、早期原肠胚、早期分割、晚期分割和孵化期斑马鱼胚胎的临界浓度分别为3.5、4、6、6和8 pM，这表明早期发育阶段的胚胎对NPs的影响比后期更敏感。有趣的是，仅在用NP处理的卵裂和早期原肠胚阶段胚胎中观察到独特的表型（头部异常和没有眼睛），显示了NP的阶段特异性效应。通过与我们对较小的Ag纳米颗粒（13.1 ± 2.5 nm）的研究比较，我们发现胚胎表型明显依赖于Ag纳米颗粒的大小和胚胎发育阶段。这些值得注意的发现表明，银纳米颗粒不同于任何常规的化学品或离子。它们可以潜在地以大小、阶段、剂量和持续时间依赖性方式实现针对早期胚胎发育的靶向特异性研究和治疗。
Nanomaterials possess distinctive physicochemical properties and promise a wide range of applications, from advanced technology to leading-edge medicine. However, their effects on living organisms remain largely unknown. Here we report that the purified silver nanoparticles (Ag NPs, 97 ± 13 nm) incite specific developmental stage embryonic phenotypes and nanotoxicity in a dose-dependent manner, upon acute exposure of given-stage embryos to the NPs (0–24 pM) for only 2 h. The critical concentrations of the NPs that cause 50% of embryos develop normally for cleavage, early-gastrula, early-segmentation, late-segmentation, and hatching stage zebrafish embryos are 3.5, 4, 6, 6, and 8 pM, respectively, showing that the earlier developmental stage embryos are much more sensitive to the effects of the NPs than the later stage. Interestingly, distinctive phenotypes (head abnormality and no eyes) are observed only in cleavage and early-gastrula stage embryos treated with the NPs, showing the stage-specific effects of the NPs. By comparing with our study of the smaller Ag NPs (13.1 ± 2.5 nm), we found that the embryonic phenotypes strikingly depend upon the sizes of Ag NPs and embryonic developmental stages. These notable findings suggest that the Ag NPs are unlike any conventional chemicals or ions. They can potentially enable target specific study and therapy for early embryonic development in size, stage, dose, and exposure-duration dependent manners.
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影响因子：
17.1
作者：
Lee, Kerry J.;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy
影响因子：
6.7
作者：
Browning LM;Lee KJ;Huang T;Nallathamby PD;Lowman JE;Xu XH
通讯作者：
Xu XH
影响因子：
4.3
作者：
Lee, Kerry J.;Browning, Lauren M.;Huang, Tao;Ding, Feng;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
使用斑马鱼胚胎和单纳米颗粒等离子体光谱研究肽功能化银纳米颗粒的电荷依赖性运输和毒性。
影响因子：
4.1
作者：
Lee KJ;Browning LM;Nallathamby PD;Xu XH
通讯作者：
Xu XH