Analysis of the mineral composition of the human calcified cartilage zone.
Analysis of the mineral composition of the human calcified cartilage zone.
复制标题
人体钙化软骨区矿物质成分分析
DOI：
10.7150/ijms.4276
复制
发表时间：
2012
影响因子：
3.6
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Zhang Y;Wang F;Tan H;Chen G;Guo L;Yang L
钙化软骨带作为透明关节软骨与软骨下骨之间的连接组织，在力的传递和材料的扩散中起着重要的作用。然而，仍有待解决的问题是其矿物组成和组织。本研究取40例健康人膝关节标本，解剖CCZ，采用番红O/固绿色染色法观察CCZ，并采用氨基酸分析法和X射线衍射法测定CCZ的化学特性。CCZ中II型胶原作为有机化合物的干重百分比为20.16% ± 0.96%，低于透明软骨层的干重百分比（61.39% ± 0.38%），羟基磷灰石作为无机化合物的干重百分比为65.09% ± 2.31%，低于软骨下骨的干重百分比（85.78% ± 3.42%）。本研究为CCZ的体外重组及结构与功能的研究提供了准确的数据。
As the connecting tissue between the hyaline articular cartilage and the subchondral bone, calcified cartilage zone (CCZ) plays a great role in the force transmission and materials diffusion. However, the questions that remain to be resolved are its mineral composition and organization. In this study, 40 healthy human knee specimens were harvested; first the CCZ was dissected and observed by Safranin O/fast green staining, then CCZ chemical characteristics were measured by using amino acid assay and X-ray diffraction. The percentage of dry weight of type II collagen as an organic compound of CCZ was 20.16% ± 0.96%, lower than that of the hyaline cartilage layer (61.39% ± 0.38%); the percentage of dry weight of hydroxyapatite as an inorganic compound was 65.09% ± 2.31%, less than that of subchondral bone (85.78% ± 3.42%). Our study provides the accurate data for the reconstruction of the CCZ in vitro and the elucidation of CCZ structure and function.
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DOI：
10.1186/ar380
发表时间：
2002
期刊：
Arthritis research
影响因子：
--
作者：
Eyre D
通讯作者：
Eyre D
影响因子：
2.8
作者：
Keinan-Adamsky, K;Shinar, H;Navon, G
通讯作者：
Navon, G
影响因子：
2.3
作者：
Lyons, Tim J.;McClure, Sheena F.;McClure, John
通讯作者：
McClure, John
影响因子：
7
作者：
Arkill, K. P.;Winlove, C. P.
通讯作者：
Winlove, C. P.
影响因子：
3
作者：
Gupta, HS;Schratter, S;Fratzl, P
通讯作者：
Fratzl, P