Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy.
Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy.
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DOI：
10.1093/nar/gkaa1013
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发表时间：
2020-12-16
影响因子：
14.9
通讯作者：
Zetzsche H
中科院分区：
文献类型：
--
作者：
Wacker A;Weigand JE;Akabayov SR;Altincekic N;Bains JK;Banijamali E;Binas O;Castillo-Martinez J;Cetiner E;Ceylan B;Chiu LY;Davila-Calderon J;Dhamotharan K;Duchardt-Ferner E;Ferner J;Frydman L;Fürtig B;Gallego J;Grün JT;Hacker C;Haddad C;Hähnke M;Hengesbach M;Hiller F;Hohmann KF;Hymon D;de Jesus V;Jonker H;Keller H;Knezic B;Landgraf T;Löhr F;Luo L;Mertinkus KR;Muhs C;Novakovic M;Oxenfarth A;Palomino-Schätzlein M;Petzold K;Peter SA;Pyper DJ;Qureshi NS;Riad M;Richter C;Saxena K;Schamber T;Scherf T;Schlagnitweit J;Schlundt A;Schnieders R;Schwalbe H;Simba-Lahuasi A;Sreeramulu S;Stirnal E;Sudakov A;Tants JN;Tolbert BS;Vögele J;Weiß L;Wirmer-Bartoschek J;Wirtz Martin MA;Wöhnert J;Zetzsche H
The current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and the 3′-untranslated region (3′-UTR) of the SCoV2 genome, their large-scale production and NMR-based secondary structure determination. The NMR data are corroborated with secondary structure probing by DMS footprinting experiments. The close agreement of NMR secondary structure determination of isolated RNA elements with DMS footprinting and NMR performed on larger RNA regions shows that the secondary structure elements fold independently. The NMR data reported here provide the basis for NMR investigations of RNA function, RNA interactions with viral and host proteins and screening campaigns to identify potential RNA binders for pharmaceutical intervention.
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影响因子：
3.7
作者：
Cho CP;Lin SC;Chou MY;Hsu HT;Chang KY
通讯作者：
Chang KY
DOI：
10.1093/bioinformatics/btp250
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Darty K;Denise A;Ponty Y
通讯作者：
Ponty Y
影响因子：
3.7
作者：
Brockway SM;Denison MR
通讯作者：
Denison MR
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
RNAstructure：用于RNA二级结构预测和分析的Web服务器。
影响因子：
14.9
作者：
Bellaousov S;Reuter JS;Seetin MG;Mathews DH
通讯作者：
Mathews DH