A new tent trap for sampling exophagic and endophagic members of the Anopheles gambiae complex.
A new tent trap for sampling exophagic and endophagic members of the Anopheles gambiae complex.
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DOI：
10.1186/1475-2875-8-157
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发表时间：
2009-07-14
期刊：
影响因子：
3
通讯作者：
Killeen GF
中科院分区：
文献类型：
--
作者：
Govella NJ;Chaki PP;Geissbuhler Y;Kannady K;Okumu F;Charlwood JD;Anderson RA;Killeen GF
蚊子采样方法对于监测和评价疟疾病媒控制干预措施至关重要。在城市达累斯萨拉姆，人类上岸渔获物（HLC）是监测疟疾传播按蚊的唯一足够敏感的方法。HLC是劳动密集型的，笨重的，危险的，并且需要如此密集的监督，难以大规模维持。开发了新型帐篷式捕集器作为HLC的替代品。Furvela帐篷是在莫桑比克设计的，采用了CDC Light trap（LT）组件，而来自坦桑尼亚Ifakara的另外两个帐篷（设计A和B）不需要电力或移动部件。采用随机拉丁方和交叉实验设计，在坦桑尼亚农村和城市环境中，分别对内食和外食人群的广泛病媒丰度范围内，将其疟疾病媒采样灵敏度与LT和HLC进行了比较。LT的敏感性大于HLC，而Ifakara帐篷陷阱的情况正好相反（An的粗平均渔获量）。对于农村环境中的设计A、B和LT，广义冈比亚相对于HLC = 0.28、0.65和1.30，对于城市环境中的设计B，广义冈比亚相对于HLC = 0.32）。然而，Ifakara B渔获量与HLC的相关性（r2 = 0.73，P < 0.001）远好于所测试的任何其他方法（Ifakara A和LT的相关性分别为r2 = 0.04，P = 0.426和r2 = 0.19，P = 0.006）。只有Ifakara B在一个高载体密度的农村环境中表现出恒定的采样效率相对于HLC。Ifakara B的相对敏感性增加，媒介密度下降，在城市环境中，并超过了HLC在最低密度。在经产蚊比例（P ≥ 0.849）或按蚊比例方面，帐篷诱捕器与HLC诱捕器无差异。冈比亚有限公司兄弟物种（P ≥ 0.280），但Ifakara A和B设计均未能降低捕获的吸血蚊子比例（比值比[95%置信区间]分别为1.6 [1.2，2.1]和1.0 [0.8，1.2]，P = 0.002和0.998），这可能是因为操作员每天早上清空诱捕器时暴露。Ifakara B诱捕器可能具有监测和评价各种内噬和外噬非洲热带疟疾病媒的潜力，特别是在低但流行病学相关的人口密度下。然而，操作员暴露于蚊虫叮咬仍然是一个问题，因此在考虑将该设计用于广泛的常规使用之前，需要进行额外的修改或采取保护措施。
Mosquito sampling methods are essential for monitoring and evaluating malaria vector control interventions. In urban Dar es Salaam, human landing catch (HLC) is the only method sufficiently sensitive for monitoring malaria-transmitting Anopheles. HLC is labour intensive, cumbersome, hazardous, and requires such intense supervision that is difficulty to sustain on large scales. Novel tent traps were developed as alternatives to HLC. The Furvela tent, designed in Mozambique, incorporates a CDC Light trap (LT) components, while two others from Ifakara, Tanzania (designs A and B) require no electricity or moving parts. Their sensitivity for sampling malaria vectors was compared with LT and HLC over a wide range of vector abundances in rural and urban settings in Tanzania, with endophagic and exophagic populations, respectively, using randomised Latin-square and cross- over experimental designs. The sensitivity of LTs was greater than HLC while the opposite was true of Ifakara tent traps (crude mean catch of An. gambiae sensu lato relative to HLC = 0.28, 0.65 and 1.30 for designs A, B and LT in a rural setting and 0.32 for design B in an urban setting). However, Ifakara B catches correlated far better to HLC (r2 = 0.73, P < 0.001) than any other method tested (r2 = 0.04, P = 0.426 and r2 = 0.19, P = 0.006 for Ifakara A and LTs respectively). Only Ifakara B in a rural setting with high vector density exhibited constant sampling efficiency relative to HLC. The relative sensitivity of Ifakara B increased as vector densities decreased in the urban setting and exceeded that of HLC at the lowest densities. None of the tent traps differed from HLC in terms of the proportions of parous mosquitoes (P ≥ 0.849) or An. gambiae s.l. sibling species (P ≥ 0.280) they sampled but both Ifakara A and B designs failed to reduce the proportion of blood-fed mosquitoes caught (Odds ratio [95% Confidence Interval] = 1.6 [1.2, 2.1] and 1.0 [0.8, 1.2], P = 0.002 and 0.998, respectively), probably because of operator exposure while emptying the trap each morning. The Ifakara B trap may have potential for monitoring and evaluating a variety of endophagic and exophagic Afrotropical malaria vectors, particularly at low but epidemiologically relevant population densities. However, operator exposure to mosquito bites remains a concern so additional modifications or protective measures will be required before this design can be considered for widespread, routine use.
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影响因子：
3
作者：
Kelly-Hope LA;McKenzie FE
通讯作者：
McKenzie FE
疟疾媒介在农村小镇和冈比亚卫星村庄的居住风险因素。
影响因子：
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作者：
Kirby, Matthew J.;Green, Clare;Milligan, Paul M.;Sismanidis, Charalambos;Jasseh, Momadou;Conway, David J.;Lindsay, Steven W.
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Lindsay, Steven W.
DOI：
10.4269/ajtmh.2005.72.725
发表时间：
2005-06-01
影响因子：
3.3
作者：
Diuk-Wasser, MA;Toure, MB;Taylor, CE
通讯作者：
Taylor, CE
DOI：
10.4269/ajtmh.2004.71.103
发表时间：
2004-08-01
影响因子：
3.3
作者：
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通讯作者：
Singer, BH
影响因子：
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作者：
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通讯作者：
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