Successful sporozoite challenge model in human volunteers with Plasmodium vivax strain derived from human donors.
Successful sporozoite challenge model in human volunteers with Plasmodium vivax strain derived from human donors.
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DOI：
10.4269/ajtmh.2009.09-0194
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发表时间：
2009-11
期刊：
影响因子：
--
通讯作者：
Arévalo-Herrera M
中科院分区：
文献类型：
--
作者：
Herrera S;Fernández O;Manzano MR;Murrain B;Vergara J;Blanco P;Palacios R;Vélez JD;Epstein JE;Chen-Mok M;Reed ZH;Arévalo-Herrera M
成功建立了间日疟原虫子孢子攻击模型。将18名健康成年人、疟疾初治志愿者随机分配到A-C组，分别暴露于感染间日疟原虫的白曼按蚊叮咬3 ± 1、6 ± 1和9 ± 1次。17名志愿者出现了与疟疾一致的体征和症状，A组的几何平均开放前期为11.1天（9.3-11）; B组为10.8天（9.8-11.9）; C组为10.6天（8.7-12.4），各组之间无统计学显著差异（Kruskal-Wallis，P = 0.70）。一名志愿者被蚊子叮咬了8次，但没有出现寄生虫血症。没有观察到寄生虫密度的差异，所有人都在抗疟疾治疗后成功康复。在18个月的随访中，没有志愿者出现寄生虫复发。总之，疟疾初治志愿者可以安全和可重复地感染叮咬2-10安。携带间日疟原虫子孢子的白蚊。该激发方法适用于疫苗和抗疟疾药物检测。
Successful establishment of a Plasmodium vivax sporozoite challenge model in humans is described. Eighteen healthy adult, malaria-naïve volunteers were randomly allocated to Groups A–C and exposed to 3 ± 1, 6 ± 1, and 9 ± 1 bites of Anopheles albimanus mosquitoes infected with P. vivax, respectively. Seventeen volunteers developed signs and symptoms consistent with malaria, and geometric mean prepatent periods of 11.1 days (9.3–11) for Group A; 10.8 days (9.8–11.9) for Group B; and 10.6 days (8.7–12.4) for Group C, with no statistically significant difference among groups (Kruskal-Wallis, P = 0.70). One volunteer exposed to eight mosquito bites did not develop a parasitemia. No differences in parasite density were observed and all individuals successfully recovered after anti-malarial treatment. None of the volunteers developed parasite relapses within an 18-month follow-up. In conclusion, malaria-naive volunteers can be safely and reproducibly infected with bites of 2–10 An. albimanus mosquitoes carrying P. vivax sporozoites. This challenge method is suitable for vaccine and anti-malarial drug testing.
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DOI：
10.1073/pnas.0406381102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Webster, DP;Dunachie, S;Hill, AVS
通讯作者：
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DOI：
10.4269/ajtmh.2001.64.97
发表时间：
2001-01-01
影响因子：
3.3
作者：
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通讯作者：
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DOI：
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发表时间：
1999-01-01
期刊：
CLINICAL AND LABORATORY HAEMATOLOGY
影响因子：
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作者：
Hänscheid, T
通讯作者：
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DOI：
10.1080/00034983.1997.11813111
发表时间：
1997-01-01
影响因子：
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作者：
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通讯作者：
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DOI：
10.1080/00034983.1984.11811851
发表时间：
1984-01-01
影响因子：
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作者：
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通讯作者：
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