Automated monitoring and analysis of social behavior in Drosophila.

Automated monitoring and analysis of social behavior in Drosophila.
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DOI:
10.1038/nmeth.1310
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发表时间:
2009-04
期刊:
影响因子:
48
通讯作者:
Perona, Pietro
Perona, Pietro
中科院分区:
生物学1区
文献类型:
--
作者:
Dankert, Heiko;Wang, Liming;Hoopfer, Eric D.;Anderson, David J.;Perona, Pietro

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介绍了一种基于机器视觉的果蝇攻击行为和求偶行为的自动测量方法。这些先天社会行为的遗传和神经回路基础知之甚少。在这种遗传上易于处理的模式生物中进行高通量行为筛选是一种潜在的强大方法,但目前非常费力。我们的系统监测相互作用的苍蝇对,并计算它们的位置,方向和翅膀姿态。这些特征用于检测攻击和求爱期间表现出的行为。其中,翅膀的威胁,弓步和扭打是特定的侵略;盘旋,翅膀延伸(求偶“歌”)和交配是特定的求偶;运动和追逐是共同的。可以根据这些测量结果自动构建行为图,节省大量时间和精力。这项技术应该能够大规模筛选控制求爱和攻击的基因和神经回路。
We introduce a method based on machine vision for automatically measuring aggression and courtship in Drosophila melanogaster. The genetic and neural circuit bases of these innate social behaviors are poorly understood. High-throughput behavioral screening in this genetically tractable model organism is a potentially powerful approach, but it is currently very laborious. Our system monitors interacting pairs of flies, and computes their location, orientation and wing posture. These features are used for detecting behaviors exhibited during aggression and courtship. Among these, wing threat, lunging and tussling are specific to aggression; circling, wing extension (courtship “song”) and copulation are specific to courtship; locomotion and chasing are common to both. Ethograms may be constructed automatically from these measurements, saving considerable time and effort. This technology should enable large-scale screens for genes and neural circuits controlling courtship and aggression.
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