The effects of dietary protein restriction on chorda tympani nerve taste responses and terminal field organization.
The effects of dietary protein restriction on chorda tympani nerve taste responses and terminal field organization.
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DOI：
10.1016/j.neuroscience.2008.09.013
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发表时间：
2008-11-19
期刊：
影响因子：
3.3
通讯作者：
Hill DL
中科院分区：
文献类型：
--
作者：
Thomas JE;Hill DL
产前饮食钠限制对大鼠功能性味觉反应和脑干神经回路的形成产生深远的发育影响。越来越多的证据表明，这些影响的潜在机制与受损的营养状态有关，而不是直接的刺激受体相互作用。我们探讨了早期营养不良是否产生类似的功能和结构的影响，通过使用蛋白质缺乏，钠充足的饮食钠限制后所看到的。为了确定早期饮食蛋白质限制是否会影响外周味觉系统的发育，在喂食蛋白质限制或对照饮食（分别为6%和20%）的大鼠中，进行了鼓索神经的多纤维神经生理学记录，并完成了鼓索神经到孤束核（NTS）的顺行追踪。饮食方案从胚胎第7天开始，并持续到大鼠用于神经生理学记录（出生后（P）35-50天）或用于鼓索终末场标记（P40-50）。对一系列浓度的NaCl、乙酸钠、KCl以及0.50 M蔗糖、0.03 M盐酸奎宁和0.01 N盐酸的反应显示，与喂食20%蛋白质饲料的大鼠相比，喂食6%蛋白质饲料的大鼠对钠特异性刺激的反应减弱（30-60%）。对所有其他刺激的反应在两组之间是相似的。终末场体积是近两倍的蛋白质限制大鼠与对照组相比，主要位于背尾区的终端领域的差异。这些结果与之前在大鼠出生前和出生后发育过程中喂养钠限制饮食的结果相似，表明饮食钠和蛋白质限制在改变味觉发育方面具有相似的机制。
Prenatal dietary sodium restriction produces profound developmental effects on rat functional taste responses and formation of neural circuits in the brainstem. Converging evidence indicates that the underlying mechanisms for these effects are related to a compromised nutritional state and not to direct stimulus-receptor interactions. We explored whether early malnourishment produces similar functional and structural effects to those seen following dietary sodium restriction by using a protein deficient, sodium replete diet. To determine if early dietary protein-restriction affects the development of the peripheral gustatory system, multi-fiber neurophysiological recordings were made from the chorda tympani nerve and anterograde track tracing of the chorda tympani nerve into the nucleus of the solitary tract (NTS) was accomplished in rats fed a protein-restricted or a control diet (6% and 20%, respectively). The dietary regimens began on embryonic day 7 and continued until rats were used for neurophysiological recordings (postnatal days (P) 35–50) or for chorda tympani terminal field labeling (P40–50). Responses to a concentration series of NaCl, sodium acetate, KCl, and to 0.50 M sucrose, 0.03 M quinine-HCl, and 0.01 N HCl revealed attenuated responses (30–60%) to sodium-specific stimuli in rats fed the 6% protein diet compared with those fed the 20% protein diet. Responses to all other stimuli were similar between groups. Terminal field volumes were nearly twofold larger in protein-restricted rats compared with controls, with the differences located primarily in the dorsal-caudal zone of the terminal field. These results are similar to the results seen previously in rats fed a sodium-restricted diet throughout pre- and postnatal development, suggesting that dietary sodium- and protein-restriction share similar mechanisms in altering gustatory development.
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影响因子：
64.5
作者：
BAKER, J;LIU, JP;EFSTRATIADIS, A
通讯作者：
EFSTRATIADIS, A
影响因子：
2.4
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56.9
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通讯作者：
DESIMONE, JA
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8
作者：
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通讯作者：
Mansilla-Olivares, A
影响因子：
56.9
作者：
Catalano, SM;Shatz, CJ
通讯作者：
Shatz, CJ