Migratory neural crest cell αN-catenin impacts chick trigeminal ganglia formation.
Migratory neural crest cell αN-catenin impacts chick trigeminal ganglia formation.
复制标题
DOI：
10.1016/j.ydbio.2014.05.016
复制
发表时间：
2014-08-15
影响因子：
2.7
通讯作者：
Taneyhill LA
中科院分区：
文献类型：
--
作者：
Wu CY;Hooper RM;Han K;Taneyhill LA
神经嵴细胞是一种胚胎细胞群，对脊椎动物的正常发育至关重要。最初定位于背神经褶皱，预迁移神经嵴细胞经历上皮-间充质转化（EMT），并迁移到发育中的胚胎中的最终目的地。神经嵴细胞与表皮源性基质细胞一起，形成周围神经系统的颅感觉神经节。我们之前的工作表明，粘附连接α-catenin蛋白的神经亚型αN-catenin通过控制前迁移神经嵴细胞钙粘蛋白水平来调节颅神经嵴细胞EMT。虽然α n-连环蛋白的下调对神经嵴细胞EMT的初始化至关重要，但α n-连环蛋白在后期神经嵴细胞迁移和颅神经节形成中的潜在作用尚未得到研究。在这项研究中，我们首次发现迁移的神经嵴细胞会产生颅三叉神经节表达αN-catenin和Cadherin-7。α n-连环蛋白丧失和功能获得实验揭示了对迁移神经嵴细胞群的影响，包括三叉神经节组装的后续缺陷。此外，αN-catenin在神经嵴细胞中的扰动影响了三叉神经节中的基板细胞，也改变了神经嵴细胞Cadherin-7的水平和定位。总之，这些结果突出了α n -连环蛋白在形成三叉神经节的迁移神经嵴细胞中的新功能。
Neural crest cells are an embryonic cell population that is crucial for proper vertebrate development. Initially localized to the dorsal neural folds, premigratory neural crest cells undergo an epithelial-tomesenchymal transition (EMT) and migrate to their final destinations in the developing embryo. Together with epidermally-derived placode cells, neural crest cells then form the cranial sensory ganglia of the peripheral nervous system. Our prior work has shown that αN-catenin, the neural subtype of the adherens junction α-catenin protein, regulates cranial neural crest cell EMT by controlling premigratory neural crest cell cadherin levels. Although αN-catenin down-regulation is critical for initial neural crest cell EMT, a potential role for αN-catenin in later neural crest cell migration, and formation of the cranial ganglia, has not been examined. In this study, we show for the first time that migratory neural crest cells that will give rise to the cranial trigeminal ganglia express αN-catenin and Cadherin-7. αN-catenin loss-and gain-of-function experiments reveal effects on the migratory neural crest cell population that include subsequent defects in trigeminal ganglia assembly. Moreover, αN-catenin perturbation in neural crest cells impacts the placode cell contribution to the trigeminal ganglia and also changes neural crest cell Cadherin-7 levels and localization. Together, these results highlight a novel function for αN-catenin in migratory neural crest cells that form the trigeminal ganglia.
登录
查看更多内容
影响因子：
4.6
作者：
Mayor, Roberto;Theveneau, Eric
通讯作者：
Theveneau, Eric
影响因子：
2.7
作者：
Steventon, Ben;Mayor, Roberto;Streit, Andrea
通讯作者：
Streit, Andrea
影响因子：
21.3
作者：
Itasaki, N;Bel-Vialar, S;Krumlauf, R
通讯作者：
Krumlauf, R
影响因子：
4.6
作者：
Shiau, Celia E.;Bronner-Fraser, Marianne
通讯作者：
Bronner-Fraser, Marianne
影响因子：
64.5
作者：
HIRANO, S;KIMOTO, N;TAKEICHI, M
通讯作者：
TAKEICHI, M