Effects of high salt stress on secondary metabolite production in the marine-derived fungus Spicaria elegans.
Effects of high salt stress on secondary metabolite production in the marine-derived fungus Spicaria elegans.
复制标题
高盐胁迫对海洋源性真菌 Spicaria elegans 次生代谢产物产生的影响
作者：
Wang Y;Lu Z;Sun K;Zhu W
为了从海洋微生物中获得结构新颖、具有生物活性的天然化合物，研究了高盐胁迫对海洋真菌Spicaria elegans KLA-03次级代谢产物产生的影响。从海洋沉积物中分离的该生物在3%和10%的盐浓度条件下培养时产生不同的次级代谢产物。分离得到4个仅在10%盐度培养条件下产生的特征代谢产物，经1D和2D NMR谱鉴定，其结构分别为（2 E，2′Z）-3，3 ′-（6，6 ′-dihydroxybiphenyl-3，3 ′-diyl）diacrylic acid（1），aspulvinone E（2），aspochalasin E（3）和aspodermamide B（6）。化合物1为新化合物。因此，高盐胁迫可能是一种有前途的手段，以诱导生产新的和氯代化合物的耐盐真菌。化合物1对铜绿假单胞菌和大肠杆菌显示出中等的抗菌活性，最小抑制浓度（MIC）值分别为0.038和0.767 mM。
To obtain structurally novel and bioactive natural compounds from marine-derived microorganisms, the effect of high salt stress on secondary metabolite production in the marine-derived fungal strain, Spicaria elegans KLA-03, was investigated. The organism, which was isolated from marine sediment, produced different secondary metabolites when cultured in 3% and 10% saline conditions. Four characteristic metabolites, only produced in the 10% salinity culture, were isolated, and their structures were identified as (2E,2′Z)-3,3′-(6,6′-dihydroxybiphenyl-3,3′-diyl)diacrylic acid (1), aspulvinone E (2), aspochalasin E (3) and trichodermamide B (6), according to their 1D and 2D NMR spectra. Compound 1 is a new compound. High salt stress may therefore be a promising means to induce the production of new and chlorinated compounds in halotolerant fungi. Compound 1 showed moderate antibacterial activity against Pseudomonas aeruginosa and Escherichia coli with minimum inhibitory concentration (MIC) values of 0.038 and 0.767 mM, respectively.
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影响因子：
9.1
作者：
Lin, Zhen Jian;Zhu, Tian Jiao;Gu, Qian Qun
通讯作者：
Gu, Qian Qun
影响因子：
5.1
作者：
Liu, Rui;Gu, Qianqun;Liu, Hongbing
通讯作者：
Liu, Hongbing
影响因子：
1.1
作者：
Lin, Zhen-Jian;Zhu, Tian-Jiao;Gu, Qian-Qun
通讯作者：
Gu, Qian-Qun
DOI：
10.1128/aem.22.2.210-213.1971
发表时间：
1971-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
TRESNER, HD;HAYES, JA
通讯作者：
HAYES, JA
异紫萼苷型生物碱，variecolorins A-L，来自耐盐曲霉
影响因子：
5.1
作者：
Wang, Wen-Liang;Lu, Zhen-Yu;Zhu, Wei-Ming
通讯作者：
Zhu, Wei-Ming