LEIGC long non-coding RNA acts as a tumor suppressor in gastric carcinoma by inhibiting the epithelial-to-mesenchymal transition.
LEIGC long non-coding RNA acts as a tumor suppressor in gastric carcinoma by inhibiting the epithelial-to-mesenchymal transition.
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牛蛙开关RGC对不同刺激持续时间的响应动态
DOI：
10.1186/1471-2407-14-932
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发表时间：
2014-12-11
期刊：
影响因子：
3.8
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Han Y;Ye J;Wu D;Wu P;Chen Z;Chen J;Gao S;Huang J
长的非编码RNA已被证明在癌症生物学中具有关键的调节作用。然而，lncRNA对胃癌的贡献在很大程度上仍然未知。采用基因芯片技术检测胃癌组织及癌旁组织中lncRNA的差异表达，并采用实时荧光定量PCR技术进行验证。进一步分析来自胃癌患者的胃样品的特异性下调的lncRNA（称为LEIGC）的水平。结果发现，胃癌组织中LEIGC的表达水平明显低于癌旁组织（P < 0.01）。过表达LEIGC可抑制肿瘤生长和细胞增殖，并增强胃癌细胞对5-氟尿嘧啶（5-FU）的敏感性，而敲低LEIGC则表现出相反的作用。我们进一步证明了LEIGC通过抑制胃癌中的上皮向间质转化（EMT）而发挥功能。我们的数据表明，LEIGC是胃癌中的肿瘤抑制lncRNA，并使我们提出lncRNA可能在癌症的发生和发展中发挥重要的调节作用。本文的在线版本（doi：10.1186/1471-2407-14-932）包含补充材料，可供授权用户使用。
Long non-coding RNAs have been shown to have critical regulatory roles in cancer biology. However, the contributions of lncRNAs to gastric cancer remain largely unknown. The differential expression of lncRNAs in gastric cancer and paired non-cancerous tissues were identified by microarray and validated using quantitative real-time PCR. Gastric samples from patients with gastric cancer were further analyzed for levels of a specifically downregulated lncRNA (termed as LEIGC). We found that there were significantly lower levels of LEIGC expression in cancer tissue than in adjacent non-cancerous tissues in human gastric cancers (P < 0.01). Overexpression of LEIGC suppressed tumor growth and cell proliferation, and enhanced the sensitivity of gastric cancer cells to 5-fluorouracil (5-FU), whereas knockdown of LEIGC showed the opposite effect. We further demonstrated LEIGC functions by inhibiting the epithelial-to-mesenchymal transition (EMT) in gastric cancer. Our data suggested that LEIGC is a tumor-suppressing lncRNA in gastric cancer, and led us to propose that lncRNAs may play important regulatory roles in cancer development and progression. The online version of this article (doi:10.1186/1471-2407-14-932) contains supplementary material, which is available to authorized users.
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影响因子：
37.3
作者：
Gibb EA;Brown CJ;Lam WL
通讯作者：
Lam WL
影响因子：
64.5
作者：
Huarte M;Guttman M;Feldser D;Garber M;Koziol MJ;Kenzelmann-Broz D;Khalil AM;Zuk O;Amit I;Rabani M;Attardi LD;Regev A;Lander ES;Jacks T;Rinn JL
通讯作者：
Rinn JL
悬浮在胶原蛋白凝胶中的上皮可以失去极性，并表达迁移间充质细胞的特征。
影响因子：
7.8
作者：
Greenburg, G;Hay, E D
通讯作者：
Hay, E D
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.2
作者：
Otsuki, Sho;Inokuchi, Mikito;Sugihara, Kenichi
通讯作者：
Sugihara, Kenichi