An aqueous media based approach for the preparation of a biosensor platform composed of graphene oxide and Pt-black.
An aqueous media based approach for the preparation of a biosensor platform composed of graphene oxide and Pt-black.
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DOI：
10.1016/j.bios.2012.06.007
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发表时间：
2012-10
影响因子：
12.6
通讯作者：
Stanciu, Lia A.
中科院分区：
文献类型：
--
作者：
Shi, Jin;Zhang, Hangyu;Snyder, Alexandra;Wang, Mei-xian;Xie, Jian;Porterfield, D. Marshall;Stanciu, Lia A.
根据之前的报道，Pt 纳米颗粒和石墨烯的组合在增强生物传感方面比单独使用任何一种纳米材料更有效。基于水溶性氧化石墨烯（GrOx）和石墨烯之间的结构相似性，我们报告了基于水介质的 GrOx/Pt-black 纳米复合材料的制造，用于增强生物传感。在这种方法中，GrOx 充当纳米级分子模板，用于电沉积 Pt-black（铂金属的非晶纳米图案同种型）。扫描电子显微镜 (SEM) 图像和能量色散 X 射线光谱 (EDS) 显示 Pt-black 沿着 GrOx 生长。 GrOx/Pt-black 微电极的有效表面积和对 H2O2 氧化的电催化活性显着高于 Pt-black 微电极。当用于制备基于用葡萄糖氧化酶 (GOx) 进行蛋白质功能化的生物纳米复合材料时，GrOx/Pt-black 微生物传感器表现出比 Pt-black 微生物传感器更高的灵敏度。这表明与单独使用铂黑相比，GrOx/铂黑纳米复合材料促进了电子转移的增加，和/或最小化了质量传输的限制。基于GrOx/Pt-black的葡萄糖微生物传感器表现出高灵敏度(465.9±48.0 nA/mM)、1 μM的低检测限、1 μM – 2 mM的线性响应范围和~4 s的响应时间。此外，该传感器非常稳定，并且对潜在干扰物具有高度选择性。
The combination of Pt nanoparticles and graphene was more effective in enhancing biosensing than either nanomaterial alone according to previous reports. Based on the structural similarities between water soluble graphene oxide (GrOx) and graphene, we report the fabrication of an aqueous media based GrOx/Pt-black nanocomposite for biosensing enhancement. In this approach GrOx acted as a nanoscale molecular template for the electrodeposition of Pt-black, an amorphously nanopatterned isoform of platinum metal. Scanning electron microscopy (SEM) images and energy-dispersive X-ray spectroscopy (EDS) showed that Pt-black was growing along GrOx. The effective surface area and electrocatalytic activity towards H2O2 oxidation of GrOx/Pt-black microelectrodes were significantly higher than for Pt-black microelectrodes. When used to prepare a bio-nanocomposite based on protein functionalization with the enzyme glucose oxidase (GOx), the GrOx/Pt-black microbiosensors exhibited improved sensitivity over the Pt-black microbiosensors. This suggested that the GrOx/Pt-black nanocomposite facilitated an increase in electron transfer, and/or minimized mass transport limitations as compared to Pt-black used alone. Glucose microbiosensors based on GrOx/Pt-black exhibited high sensitivity (465.9±48.0 nA/mM), a low detection limit of 1 μM, a linear response range of 1 μM – 2 mM, and response time of ~4 s. Additionally the sensor was stable and highly selective over potential interferents.
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影响因子：
5.4
作者：
Kohma, Takuya;Oyamatsu, Daisuke;Kuwabata, Susumu
通讯作者：
Kuwabata, Susumu
影响因子：
3
作者：
McLamore, E. S.;Mohanty, S.;Porterfield, D. M.
通讯作者：
Porterfield, D. M.
影响因子：
7.4
作者：
MELL, LD;MALOY, JT
通讯作者：
MALOY, JT
影响因子：
4.3
作者：
Pumera, Martin;Scipioni, Roberto;Boero, Mauro
通讯作者：
Boero, Mauro
影响因子：
16.6
作者：
Rourke, Jonathan P.;Pandey, Priyanka A.;Wilson, Neil R.
通讯作者：
Wilson, Neil R.