The Cryptosporidium parvum ApiAP2 gene family: insights into the evolution of apicomplexan AP2 regulatory systems.
The Cryptosporidium parvum ApiAP2 gene family: insights into the evolution of apicomplexan AP2 regulatory systems.
复制标题
parvum parvum apiap2基因家族：对Apicomplexan AP2调节系统的演变的见解。
DOI：
10.1093/nar/gku500
复制
发表时间：
2014-07
影响因子：
14.9
通讯作者：
Kissinger JC
中科院分区：
文献类型：
--
作者：
Oberstaller J;Pumpalova Y;Schieler A;Llinás M;Kissinger JC
我们首次对隐孢子虫中的转录因子家族进行了全面的分析，隐孢子虫是一种基础分枝顶端复合体，是全球婴儿腹泻的第二大原因。含AP2结构域的蛋白已经进化为该门中的主要调控家族，排除了典型的调控因子。我们发现顶端复合体和Perkinsid AP2结构域与其他染色质AP2结构域有明显的聚集性。微小隐孢子虫AP2结构域的蛋白结合特异性分析与共调控基因簇上游的基序保守相结合，允许在体外生命周期中构建假定的AP2调节子。与恶性疟原虫相比，同源Apicomplexan AP2(ApiAP2)的表达发生了重排，提示ApiAP2网络在进化过程中发生了重新布线。人类微小隐孢子虫ApiAP2蛋白和靶基因的同源基因显示出比平均水平更大的变异。与恶性疟原虫相比，微小疟原虫AP2结构域的结合多样性降低，多个结构域与5‘-TGCAT-3’，5‘-CACACA-3’和G-box基序(5‘-G[T/C]GGGG-3’)结合。在微小隐孢子虫上游区域，许多过度表达的基序不是AP2结合基序。我们认为，微小隐孢子虫对ApiAP2调节剂的依赖程度较低，部分原因是它利用了E2F/DP1转录因子。微小隐孢子虫可能为顶端复合体转录调控的祖先状态提供线索，即AP2之前的统治。
We provide the first comprehensive analysis of any transcription factor family in Cryptosporidium, a basal-branching apicomplexan that is the second leading cause of infant diarrhea globally. AP2 domain-containing proteins have evolved to be the major regulatory family in the phylum to the exclusion of canonical regulators. We show that apicomplexan and perkinsid AP2 domains cluster distinctly from other chromalveolate AP2s. Protein-binding specificity assays of C. parvum AP2 domains combined with motif conservation upstream of co-regulated gene clusters allowed the construction of putative AP2 regulons across the in vitro life cycle. Orthologous Apicomplexan AP2 (ApiAP2) expression has been rearranged relative to the malaria parasite P. falciparum, suggesting ApiAP2 network rewiring during evolution. C. hominis orthologs of putative C. parvum ApiAP2 proteins and target genes show greater than average variation. C. parvum AP2 domains display reduced binding diversity relative to P. falciparum, with multiple domains binding the 5′-TGCAT-3′, 5′-CACACA-3′ and G-box motifs (5′-G[T/C]GGGG-3′). Many overrepresented motifs in C. parvum upstream regions are not AP2 binding motifs. We propose that C. parvum is less reliant on ApiAP2 regulators in part because it utilizes E2F/DP1 transcription factors. C. parvum may provide clues to the ancestral state of apicomplexan transcriptional regulation, pre-AP2 domination.
登录
查看更多内容
影响因子：
3.7
作者：
Behnke MS;Wootton JC;Lehmann MM;Radke JB;Lucas O;Nawas J;Sibley LD;White MW
通讯作者：
White MW
影响因子：
4.3
作者：
Altschul SF;Wootton JC;Zaslavsky E;Yu YK
通讯作者：
Yu YK
DOI：
10.1126/science.1162327
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Badis G;Berger MF;Philippakis AA;Talukder S;Gehrke AR;Jaeger SA;Chan ET;Metzler G;Vedenko A;Chen X;Kuznetsov H;Wang CF;Coburn D;Newburger DE;Morris Q;Hughes TR;Bulyk ML
通讯作者：
Bulyk ML
影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
影响因子：
6.7
作者：
Campbell TL;De Silva EK;Olszewski KL;Elemento O;Llinás M
通讯作者：
Llinás M