Three-dimensional vizualization of mouse brain by lipid analysis using ambient ionization mass spectrometry.
Three-dimensional vizualization of mouse brain by lipid analysis using ambient ionization mass spectrometry.
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DOI：
10.1002/anie.200906283
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发表时间：
2010
影响因子：
16.6
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
--
作者：
Eberlin, Livia S.;Ifa, Demian R.;Wu, Chunping;Cooks, R. Graham
二维 (2D) 成像质谱 (MS)[1] 已成为生物科学领域的一项强大技术。它可以以高特异性直接研究植物和动物组织中各种脂质、药物、生物防御剂、色素和蛋白质的分布，无需组织化学方案中通常使用的荧光或放射性标记。[2, 3] 在成像 MS 中，将表面上存在的分子的化学特性作为其 2D 空间分布（x 和 y 坐标）的函数进行研究。三维 (3D) 图像可以从一组合适的 2D 数据构建，它们说明了空间特定生物分子在组织亚结构中的分布，包括病理特征[4]。描述了两种基本方法，即深度分析和连续切片，用于记录创建基于 3D MS 的图像所需的信息。在深度剖析实验中，解吸剂用于在记录表面分析数据之间从物体表面去除浅层材料。二次离子质谱 (SIMS)[5]、激光烧蚀电喷雾电离 (LAESI)[2, 6] 和激光烧蚀流动大气压余辉 (LA-FAPA)[7] 是已用于通过深度剖析创建 3D MS 模型的电离技术。在替代的连续切片方法中，通过机械切片获得物体的连续切片并单独分析。基质辅助激光解吸电离 (MALDI-MS)[8] 是唯一用于 3D 成像连续切片分析的技术。
Two-dimensional (2D) imaging mass spectrometry (MS)[1] has emerged as a powerful technique in the biological sciences. It allows direct investigation of the distribution of a variety of lipids, drugs, biological defensive agents, pigments and proteins in plant and animal tissues with high specificity and without the need of fluorescent or radioactive labelling normally used in histochemical protocols.[2, 3] In imaging MS, the chemical identity of molecules present on a surface is investigated as a function of their 2D spatial distribution (x and y coordinates).Three-dimensional (3D) images can be constructed from a suitable set of 2D data and they illustrate the spatial distributions of specific biomolecules in substructures of tissues including pathological features [4]. Two basic approaches, depth profiling and serial sectioning, have been described for recording the information needed to create 3D MS-based images. In depth profiling experiments, the desorbing agent is used to remove shallow layers of material from the surface of the object in between recording surface analysis data. Secondary ion mass spectrometry (SIMS)[5], laser ablation electrospray ionization (LAESI)[2, 6] and laser ablation flowing atmospheric-pressure afterglow (LA-FAPA)[7] are ionization techniques that have been used to create 3D MS models by depth profiling. In the alternative serial sectioning approach, serial sections of the object are obtained through mechanical sectioning and individually analyzed. Matrix assisted laser desorption ionization (MALDI-MS)[8] is the only technique that have been used to serial sectioning analysis for 3D imaging.
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影响因子：
7.4
作者：
Shelley, Jacob T.;Ray, Steven J.;Hieftje, Gary M.
通讯作者：
Hieftje, Gary M.
影响因子：
2
作者：
Kauppila, Tiina J.;Talaty, Nari;Cooks, R. Graham
通讯作者：
Cooks, R. Graham
影响因子：
7.4
作者：
Wu, Chunping;Ifa, Demian R.;Manicke, Nicholas E.;Cooks, R. Graham
通讯作者：
Cooks, R. Graham
影响因子：
7.4
作者：
Chaurand, P;Schwartz, SA;Caprioli, RM
通讯作者：
Caprioli, RM
DOI：
10.1016/j.jasms.2007.12.003
发表时间：
2008-04-01
影响因子：
3.2
作者：
Manicke, Nicholas E.;Wiseman, Justin M.;Cooks, R. Graham
通讯作者：
Cooks, R. Graham