Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy.
Menopause induces changes to the stratum corneum ceramide profile, which are prevented by hormone replacement therapy.
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DOI：
10.1038/s41598-022-26095-0
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发表时间：
2022-12-15
影响因子：
4.6
通讯作者：
Nicolaou, Anna
中科院分区：
文献类型：
--
作者：
Kendall, Alexandra C.;Pilkington, Suzanne M.;Wray, Jonathan R.;Newton, Victoria L.;Griffiths, Christopher E. M.;Bell, Mike;Watson, Rachel E. B.;Nicolaou, Anna
绝经期可导致表皮变化，可通过激素替代疗法（HRT）缓解。我们假设这些变化可能与神经酰胺的产生有关，雌激素可能在角质形成细胞神经酰胺代谢中起作用。将白色白种人妇女分为三组：绝经前（n = 7）、绝经后（n = 11）和绝经后接受HRT（n = 10）。评估血液样品的激素水平，测量经表皮水分损失以评估皮肤屏障功能，并从光保护的角质层皮肤中取样角质层脂质。神经酰胺和鞘磷脂进行了分析，超高效液相色谱电喷雾电离和串联质谱。绝经后角质层含有较低水平的神经酰胺，平均长度较短; HRT组的变化不明显。血清雌二醇与神经酰胺丰度和长度相关。神经酰胺具有较短的鞘氨醇碱基，表明神经酰胺的从头生物合成发生了改变。此外，绝经后女性的鞘磷脂水平较高，表明可能对水解途径产生影响。用雌二醇（10 nM）处理原代人角质形成细胞增加了CER[NS]和CER[NDS]神经酰胺的产生，证实了雌激素对皮肤神经酰胺代谢的影响。总而言之，这些数据表明绝经后角质层脂质受到干扰，以及雌激素在神经酰胺产生中的作用。
The menopause can lead to epidermal changes that are alleviated by hormone replacement therapy (HRT). We hypothesise that these changes could relate to altered ceramide production, and that oestrogen may have a role in keratinocyte ceramide metabolism. White Caucasian women were recruited into three groups: pre-menopausal (n = 7), post-menopausal (n = 11) and post-menopausal taking HRT (n = 10). Blood samples were assessed for hormone levels, transepidermal water loss was measured to assess skin barrier function, and stratum corneum lipids were sampled from photoprotected buttock skin. Ceramides and sphingomyelins were analysed by ultraperformance liquid chromatography with electrospray ionisation and tandem mass spectrometry. Post-menopausal stratum corneum contained lower levels of ceramides, with shorter average length; changes that were not evident in the HRT group. Serum oestradiol correlated with ceramide abundance and length. Ceramides had shorter sphingoid bases, indicating altered de novo ceramide biosynthesis. Additionally, post-menopausal women had higher sphingomyelin levels, suggesting a possible effect on the hydrolysis pathway. Treatment of primary human keratinocytes with oestradiol (10 nM) increased production of CER[NS] and CER[NDS] ceramides, confirming an effect of oestrogen on cutaneous ceramide metabolism. Taken together, these data show perturbed stratum corneum lipids post-menopause, and a role for oestrogen in ceramide production.
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影响因子：
4.9
作者：
Affinito, P;Palomba, S;Nappi, C
通讯作者：
Nappi, C
DOI：
10.1073/pnas.0811269106
发表时间：
2009-05-19
影响因子：
11.1
作者：
Han, Gongshe;Gupta, Sita D.;Dunn, Teresa M.
通讯作者：
Dunn, Teresa M.
影响因子：
6.5
作者：
Janssens, Michelle;van Smeden, Jeroen;Bouwstra, Joke A.
通讯作者：
Bouwstra, Joke A.
影响因子：
3.6
作者：
Emmert, Hila;Baurecht, Hansjoerg;Weidinger, Stephan
通讯作者：
Weidinger, Stephan
影响因子：
4.5
作者：
Cho Y;Lew BL;Seong K;Kim NI
通讯作者：
Kim NI