Automated video analysis system reveals distinct diurnal behaviors in C57BL/6 and C3H/HeN mice.
Automated video analysis system reveals distinct diurnal behaviors in C57BL/6 and C3H/HeN mice.
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DOI：
10.1016/j.bbr.2013.01.003
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发表时间：
2013-04-15
影响因子：
2.7
通讯作者：
Dubocovich, M. L.
中科院分区：
文献类型：
--
作者：
Adamah-Biassi, E. B.;Stepien, I.;Hudson, R. L.;Dubocovich, M. L.
啮齿动物行为解剖的进展，使用自动视频记录和分析允许详细的表型。本研究比较和对比了15个昼夜行为连续记录使用自动行为分析系统在14/10光/暗周期在单独圈养的C3 H/HeN（C3 H）或C57 BL/6（C57）雄性小鼠为期14天。以最小的实验干扰记录并使用表型阵列和时间分布分析进行分析的昼夜行为分别在C57和C3 H小鼠中显示双峰和单峰曲线。表型阵列分析揭示了C3 H和C57小鼠的活动样行为（即行走、悬挂、跳跃、下来）（ALB）、探索样行为（即挖掘、梳理、竖起、嗅闻、伸展）（ELB）、摄取样行为（即饮水、进食）（ILB）和休息样行为（即清醒、保持低、休息、抽搐）（RLB）中的不同行为节律。时间分析表明，压力和一天中的时间会影响自发居家行为的大小和分布。轮运行活动，水和食物的测量与居家行为的时间。皮下（3毫克/公斤，sc）或口服（0.02毫克/毫升，口服）褪黑激素治疗C57小鼠没有修改无论是总的24小时大小或时间分布的家居行为时，与车辆治疗。我们的结论是，C3 H和C57小鼠表现出不同的自发活动和行为节律，特别是在夜间不调制的褪黑激素。
Advances in rodent behavior dissection using automated video recording and analysis allows detailed phenotyping. This study compared and contrasted 15 diurnal behaviors recorded continuously using an automated behavioral analysis system for a period of 14 days under a 14/10 light/dark cycle in single housed C3H/HeN (C3H) or C57BL/6 (C57) male mice. Diurnal behaviors, recorded with minimal experimental interference and analyzed using phenotypic array and temporal distribution analysis showed bimodal and unimodal profiles in the C57 and C3H mice, respectively. Phenotypic array analysis revealed distinct behavioral rhythms in activity-like behaviors (i.e. walk, hang, jump, come down) (ALB), exploration-like behaviors (i.e. dig, groom, rear up, sniff, stretch) (ELB), ingestion-like behaviors (i.e. drink, eat) (ILB) and resting-like behaviors (i.e. awake, remain low, rest, twitch) (RLB) of C3H and C57 mice. Temporal analysis demonstrated that strain and time of day affects the magnitude and distribution of the spontaneous homecage behaviors. Wheel running activity, water and food measurements correlated with timing of homecage behaviors. Subcutaneous (3 mg/kg, sc) or oral (0.02 mg/ml, oral) melatonin treatments in C57 mice did not modify either the total 24 hr magnitude or temporal distribution of homecage behaviors when compared with vehicle treatments. We conclude that C3H and C57 mice show different spontaneous activity and behavioral rhythms specifically during the night period which are not modulated by melatonin.
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影响因子：
3.5
作者：
Benloucif, S;Dubocovich, ML
通讯作者：
Dubocovich, ML
影响因子：
2.9
作者：
Daniels, DM;Stoddart, CW;Rakoczy, PE
通讯作者：
Rakoczy, PE
影响因子：
1.8
作者：
Kim, Ki Hean;Puoris'haag, Mehron;de Boer, Johannes F.
通讯作者：
de Boer, Johannes F.
影响因子：
2.7
作者：
Fonken, Laura K.;Finy, M. Sima;Nelson, Randy J.
通讯作者：
Nelson, Randy J.
影响因子：
2.6
作者：
Bachmanov, AA;Reed, DR;Tordoff, MG
通讯作者：
Tordoff, MG