Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3′ Untranslated Region of nanos3 or vasa Gene
Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3′ Untranslated Region of nanos3 or vasa Gene
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使用由 nanos3 或 vasa 基因 3 英寸非翻译区介导的荧光蛋白对大菱鲆（大菱鲆）原始生殖细胞进行体内可视化
DOI：
10.1007/s10126-019-09911-z
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发表时间：
2019-09
期刊：
影响因子：
--
通讯作者：
Jun Li
中科院分区：
文献类型：
--
作者：
Li Zhou;Xueying Wang;Qinghua Liu;Shihong Xu;Haixia Zhao;Mingming Han;Yunong Wang;Zongcheng Song;Jun Li
原始生殖细胞（Primitive germ cells，PGCs）是生殖细胞的前体细胞，负责将遗传信息传递给下一代。硬骨鱼类原始生殖细胞的活体可视化研究对于研究生殖细胞的起源和发育，以及进一步进行原始生殖细胞的分离、冷冻保存和替代育种等具有重要意义。本研究将荧光蛋白编码区与纳米3 orvasa基因3′非翻译区（3′UTR）融合构建的人工合成mRNAs（mCherry-Smnanos 33 ′UTR或mCherry-Smvasa 3 ′UTR mRNA）注射大菱鲆（Scophthalmus maximus）受精卵，用于追踪PGCs。结果表明，荧光PGCs由体细胞分化而成，在胚胎发育早期排列于躯干两侧，随后迁移至肠背侧，在此部位形成性腺。同样，我们还发现斑马鱼vasa 3 ′UTR可以标记大菱鲆PGCs，而青鳉和真鲷的vasa 3 ′UTR虽然可以标记青鳉PGCs，但不能标记。此外，通过比较分析，我们发现nanos 3和vasa的3 ′ UTR中的一些潜在序列元件，如GCAC、62-bp的富U区和核苷酸187-218区可能参与PGCs的稳定。本研究结果提供了一种高效、快速、特异的非转基因方法，可用于经济鱼类PGCs的活体检测。
Primordial germ cells (PGCs) as the precursors of germ cells are responsible for transmitting genetic information to the next generation. Visualization of teleost PGCs in vivo is essential to research the origination and development of germ cells and facilitate further manipulation on PGCs isolation, cryopreservation, and surrogate breeding. In this study, artificially synthesized mRNAs constructed by fusing fluorescent protein coding region to the 3′ untranslated region (3′UTR) ofnanos3orvasa(mCherry-Smnanos33′UTR or mCherry-Smvasa3′UTR mRNA) were injected into turbot (Scophthalmus maximus) fertilized eggs for tracing PGCs. The results demonstrated that the fluorescent PGCs differentiated from somatic cells and aligned on both sides of the trunk at the early segmentation period, then migrated and located at the dorsal part of the gut where the gonad would form. In the same way, we also found that the zebrafish (Danio rerio)vasa3′UTR could trace turbot PGCs, while thevasa3′UTR s of marine medaka (Oryzias melastigma) and red seabream (Pagrus major) failed, although they could label the marine medaka PGCs. In addition, through comparative analysis, we discovered that some potential sequence elements in the3 ′UTRs ofnanos3andvasa, such as GCACs, 62-bp U-rich regions and nucleotide 187–218 regions might be involved in PGCs stabilization. The results of this study provided an efficient, rapid, and specific non-transgenic approach for visualizing PGCs of economical marine fish in vivo.
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影响因子：
3
作者：
Adrijana Skugor;Krasimir Slanchev;J. Torgersen;H. Tveiten;Ø. Andersen
通讯作者：
Adrijana Skugor;Krasimir Slanchev;J. Torgersen;H. Tveiten;Ø. Andersen
使用 Nanos3 鉴定了橄榄鲽 (Paralichthys olivaceus) 原始生殖细胞迁移的新模式
影响因子：
2.4
作者：
Wang, Qian;Wu, Zhihao;You Feng;Zou, Yuxia
通讯作者：
Zou, Yuxia
影响因子：
4.6
作者：
Zhou L;Feng Y;Wang F;Dong X;Jiang L;Liu C;Zhao Q;Li K
通讯作者：
Li K
DOI：
10.1387/ijdb.10668983
发表时间：
1999
期刊：
The International journal of developmental biology
影响因子：
--
作者：
A. K. Braat;J. Speksnijder;Danica Zivkovic
通讯作者：
A. K. Braat;J. Speksnijder;Danica Zivkovic
影响因子：
2.6
作者：
Li, Mingyou;Hong, Ni;Hong, Yunhan
通讯作者：
Hong, Yunhan