The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study.
The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study.
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DOI：
10.3390/biom12081160
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发表时间：
2022-08-21
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究的目的是量化ESKAPEE细菌，编码针对这组病原体的抗生素抗性的基因，以及城市污水和河水中的整合酶基因。从收集的样品中提取环境DNA，并用Illumina TruSeq试剂盒进行深度测序。确定了属于ESKAPEE组的细菌属和种的丰度、与该微生物组相关的400个ARG和三类整合酶基因。分类学分析表明，不动杆菌属是优势菌属，而鲍曼不动杆菌和大肠杆菌是优势菌种。通过以下ARG的最高浓度对分析样本进行表征：blaGES、blaOXA-58、blaTEM、qnrB和qnrS。鲍曼不动杆菌、E.大肠杆菌，并检测编码耐β-内酰胺类（blaVEB-1，blaIMP-1，blaGES，blaOXA-58，blaCTX-M，blaTEM）和氟喹诺酮类（qnrS）的基因。相关分析表明，A.鲍曼不动杆菌（被认为是新兴的人类病原体的细菌物种）和编码对所有测试的抗菌剂组的抗性的基因。研究的细菌（特别是A.鲍曼不动杆菌）和ARGs对水生环境构成公共卫生风险。
The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera and species belonging to the ESKAPEE group, 400 ARGs associated with this microbial group, and three classes of integrase genes were determined. A taxonomic analysis revealed that Acinetobacter was the dominant bacterial genus, whereas Acinetobacter baumannii and Escherichia coli were the dominant bacterial species. The analyzed samples were characterized by the highest concentrations of the following ARGs: blaGES, blaOXA-58, blaTEM, qnrB, and qnrS. Acinetobacter baumannii, E. coli, and genes encoding resistance to β-lactams (blaVEB-1, blaIMP-1, blaGES, blaOXA-58, blaCTX-M, and blaTEM) and fluoroquinolones (qnrS) were detected in samples of river water collected downstream from the wastewater discharge point. The correlation analysis revealed a strong relationship between A. baumannii (bacterial species regarded as an emerging human pathogen) and genes encoding resistance to all tested groups of antimicrobials. The transmission of the studied bacteria (in particular A. baumannii) and ARGs to the aquatic environment poses a public health risk.
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影响因子：
3.9
作者：
Amin, Mansour;Navidifar, Tahereh;Goodarzi, Hamed
通讯作者：
Goodarzi, Hamed
DOI：
10.1186/s12941-015-0100-6
发表时间：
2015-10-20
影响因子：
5.7
作者：
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影响因子：
3.7
作者：
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通讯作者：
Nelson DC
影响因子：
11.8
作者：
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通讯作者：
Bartlett, John
影响因子：
11
作者：
Barberan, Albert;Bates, Scott T.;Fierer, Noah
通讯作者：
Fierer, Noah