A microfluidics cytometer for mice anemia detection.

A microfluidics cytometer for mice anemia detection.
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用于小鼠贫血检测的微流控细胞仪。

DOI:
10.1039/c2lc40332c
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发表时间:
2012-10
期刊:
影响因子:
6.1
通讯作者:
Zhihong Li
Zhihong Li
中科院分区:
工程技术1区
文献类型:
--
作者:
Wei Wang;Lide Xie;Wei Juan Yao;Zhihong Li

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介绍了一种微流控流式细胞仪系统的设计、制作及其在网织红细胞检测中的应用。该芯片可以对靶细胞进行计数,靶细胞聚焦在检测窗口,没有鞘流。这种基于优化落射荧光的细胞仪系统在信噪比方面具有竞争优势。与抗体结合的网织红细胞的诱导荧光由光学模块检测,然后由光电倍增管转换成电信号。信号处理后,结果由数字模块自动读出并显示在系统上。为了评价该微流控细胞仪系统,分别采用聚苯乙烯(PS)微珠和诱导的贫血小鼠网织红细胞进行实验,结果表明该微流控细胞仪系统可有效检测网织红细胞。
The design and fabrication of a microfluidic cytometer system and its application for reticulocyte detection are described. This chip can count the target cells, which are focused at the detection window without sheath flow. This cytometer system based on optimized epifluoresence has a competitive advantage in the signal-to-noise ratio. Induced fluorescence from the reticulocyte binded with antibody is detected by the optical module and then transformed into the electronic signal by a photo multiplier tube. After signal processing, the results are automatically read out by a digital module and displayed on the system. To evaluate this microfluidic cytometer system, experiments employing polystyrene (PS) micro beads and induced reticulocyte of mice anemia are carried out, respectively, and the results illustrate that the microfluidic cytometer system is effective in detecting the reticulocyte.
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发表时间: 2001-05
影响因子: 1.3
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