Microtubular dynamics in granulosa cells of periovulatory follicles and granulosa-derived (large) lutein cells of sheep: relationships to the steroidogenic folliculo-luteal shift and functional luteolysis.
Microtubular dynamics in granulosa cells of periovulatory follicles and granulosa-derived (large) lutein cells of sheep: relationships to the steroidogenic folliculo-luteal shift and functional luteolysis.
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绵羊围排卵卵泡颗粒细胞和颗粒衍生（大）叶黄素细胞的微管动力学：与类固醇生成卵泡黄体转移和功能性黄体溶解的关系。
作者：
W. Murdoch
间接免疫荧光光学显微镜用于监测的时间扰动的颗粒/黄体细胞在石蜡包埋切片的排卵期卵泡和黄体的羊的微管（微管蛋白）系统。尚未暴露于促性腺激素峰的排卵前卵泡的雌激素活性颗粒细胞微管蛋白免疫染色强烈。激增开始后，微管基质的免疫染色减弱，与滤泡雌二醇产生的突然下降同时发生。一个短暂的微管收缩期的特点是低水平的类固醇激素输出。随着排卵的临近和卵泡孕酮生物合成的增加（黄体化），微管再次出现。在排卵前期用秋水仙碱（一种与微管蛋白特异性结合并干扰微管组装的药物）治疗动物，阻碍了卵泡向孕酮的转变。微管动力学（聚合/<->解聚）明显支持卵泡类固醇生成的基本机制。最后，在发情周期第10天前列腺素（PG）F2 α给药前（0 h）和给药后，从母羊中分离黄体。黄体孕酮浓度在2 h时略有增加，随后从4 h至16 h急剧下降。24小时后，黄体重量减少（结构回归）。对照黄体的大（PG敏感）类固醇生成细胞的切片通常显示放射状微管网络。PGF 2 α给药后，大细胞的管状基质缺乏;透射电子显微镜下线粒体聚集明显。大的黄体细胞的细胞骨架的附着解体可能是迄今为止未被认识的事件在生物力学的功能性黄体溶解-也许解偶联胆固醇易位线粒体细胞色素P450侧链裂解。
Indirect immunofluorescence light microscopy was used to monitor temporal perturbations in the microtubular (tubulin) system of granulosa/lutein cells in paraffin-embedded sections of periovulatory follicles and corpora lutea of sheep. Estrogen-active granulosa cells of preovulatory follicles not yet exposed to the gonadotropin surge immunostained intensely for tubulin. Immunostaining of the microtubular matrix diminished after the onset of the surge and coincident with an abrupt fall in follicular estradiol production. A transient period of microtubular retraction was characterized by low-level steroid hormone output. Microtubules reappeared with the approach of ovulation and increase in follicular progesterone biosynthesis (luteinization). Treatment of animals during the preovulatory period with colchicine, a drug that binds specifically with tubulin and interferes with microtubular assembly, obstructed the follicular shift toward progesterone. Microtubular dynamics (polymerization<->depolymerization) evidently underpin fundamental mechanisms of follicular steroidogenesis. Finally, corpora lutea were isolated from ewes on Day 10 of the estrous cycle before (0 h) and after administration of prostaglandin (PG) F2 alpha. There was a small augmentation in luteal concentrations of progesterone at 2 h, followed by a sharp decrease from 4 to 16 h. Luteal weights were reduced (structural regression) at 24 h. Sections of large (PG-sensitive) steroidogenic cells of control corpora lutea typically displayed a radiating microtubular network. After administration of PGF2 alpha, tubular matrices of large cells were scant; mitochondrial clustering was evident in transmission electron micrographs. Affixed disassembly of the cytoskeleton of large luteal cells may be a heretofore unrecognized event in the biomechanics of functional luteolysis--perhaps uncoupling cholesterol translocation to mitochondrial cytochrome P450 side-chain cleavage.
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影响因子：
20.3
作者：
J. Richards
通讯作者：
J. Richards
哺乳动物排卵期间可能影响卵巢滤泡细胞膜电位的因素综述。
DOI：
10.1530/acta.0.126s0np
发表时间：
1992
期刊：
Acta endocrinologica
影响因子：
--
作者：
Espey,LL
通讯作者：
Espey,LL
小和大绵羊黄体细胞中游离细胞内钙浓度的激素调节。
影响因子：
3.6
作者：
Wiltbank,MC;Guthrie,PB;Mattson,MP;Kater,SB;Niswender,GD
通讯作者：
Niswender,GD
真核细胞中的脂质运输过程。
DOI：
10.1016/0005-2760(94)00073-5
发表时间：
1994
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Trotter,PJ;Voelker,DR
通讯作者：
Voelker,DR
促性腺激素和环腺苷 3,5-单磷酸 (cAMP) 会改变培养的人颗粒细胞的形态。
影响因子：
3.6
作者：
Soto,EA;Kliman,HJ;Strauss3rd,JF;Paavola,LG
通讯作者：
Paavola,LG