Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs.
Parenteral Fish-Oil Containing Lipid Emulsions Limit Initial Lipopolysaccharide-Induced Host Immune Responses in Preterm Pigs.
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DOI：
10.3390/nu13010205
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发表时间：
2021-01-12
期刊：
影响因子：
5.9
通讯作者：
Martin CR
中科院分区：
文献类型：
--
作者：
Yakah W;Ramiro-Cortijo D;Singh P;Brown J;Stoll B;Kulkarni M;Oosterloo BC;Burrin D;Maddipati KR;Fichorova RN;Freedman SD;Martin CR
多组分脂质乳剂可用于早产儿的重症监护。我们试图确定不同脂质乳剂对宿主的早期启动及其对急性刺激的反应的影响。早产7d的猪（n = 59）随机接受不同的脂质乳剂，为期11 d: 100%大豆油（SO）、混合油乳剂（SO、中链橄榄油和鱼油）（含15%鱼油（MO15））或100%鱼油（FO100）。第11天，猪接受8小时连续静脉输注脂多糖（lps -冻干大肠杆菌）或生理盐水。收集血浆进行脂肪酸、氧脂、代谢组学和细胞因子分析。在第11天，FO100组的血浆omega-3脂肪酸水平显示出二十碳五烯酸、EPA（0.1±0.0至9.7±1.9,p < 0.001）、二十二碳六烯酸、DHA（第0天= 2.5±0.7至13.6±2.9,p < 0.001）、EPA和DHA衍生的氧磷脂以及鞘磷脂代谢物的最高水平。在SO组，细胞因子il - 1β水平在LPS输注的第一个小时升高（296.6±308 pg/mL），但在MO15， FO100或接受生理盐水代替LPS的动物中未检测到。大豆油组猪在第1小时花生四烯酸（AA）来源的前列腺素和血栓素含量显著升高（p < 0.05）。在LPS输注期间，两组小鼠的氧脂素均未见明显变化。在产后早期注入大豆油的寄主保持了较高的AA:DHA比例和对外部刺激的急性反应能力。相比之下，含有鱼油的脂质乳剂增加了DHA，加剧了AA的缺陷，并限制了早产儿lps诱导的初始炎症反应。
Multicomponent lipid emulsions are available for critical care of preterm infants. We sought to determine the impact of different lipid emulsions on early priming of the host and its response to an acute stimulus. Pigs delivered 7d preterm (n = 59) were randomized to receive different lipid emulsions for 11 days: 100% soybean oil (SO), mixed oil emulsion (SO, medium chain olive oil and fish oil) including 15% fish oil (MO15), or 100% fish oil (FO100). On day 11, pigs received an 8-h continuous intravenous infusion of either lipopolysaccharide (LPS—lyophilized Escherichia coli) or saline. Plasma was collected for fatty acid, oxylipin, metabolomic, and cytokine analyses. At day 11, plasma omega-3 fatty acid levels in the FO100 groups showed the highest increase in eicosapentaenoic acid, EPA (0.1 ± 0.0 to 9.7 ± 1.9, p < 0.001), docosahexaenoic acid, DHA (day 0 = 2.5 ± 0.7 to 13.6 ± 2.9, p < 0.001), EPA and DHA-derived oxylipins, and sphingomyelin metabolites. In the SO group, levels of cytokine IL1β increased at the first hour of LPS infusion (296.6 ± 308 pg/mL) but was undetectable in MO15, FO100, or in the animals receiving saline instead of LPS. Pigs in the SO group showed a significant increase in arachidonic acid (AA)-derived prostaglandins and thromboxanes in the first hour (p < 0.05). No significant changes in oxylipins were observed with either fish-oil containing group during LPS infusion. Host priming with soybean oil in the early postnatal period preserves a higher AA:DHA ratio and the ability to acutely respond to an external stimulus. In contrast, fish-oil containing lipid emulsions increase DHA, exacerbate a deficit in AA, and limit the initial LPS-induced inflammatory responses in preterm pigs.
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影响因子：
6.7
作者：
Alba-Loureiro, TC;Martins, EF;Sannomiya, P
通讯作者：
Sannomiya, P
DOI：
10.1002/jpen.1692
发表时间：
2020-05
期刊：
JPEN. Journal of parenteral and enteral nutrition
影响因子：
--
作者：
Belza C;Wales JC;Courtney-Martin G;de Silva N;Avitzur Y;Wales PW
通讯作者：
Wales PW
影响因子：
5.9
作者：
Hadley KB;Ryan AS;Forsyth S;Gautier S;Salem N Jr
通讯作者：
Salem N Jr
影响因子：
3.4
作者：
Akinsulire, Olajumoke;Perides, George;Martin, Camilia R.
通讯作者：
Martin, Camilia R.
影响因子：
7.1
作者：
Carnielli, Virgilio P.;Simonato, Manuela;Cogo, Paola E.
通讯作者：
Cogo, Paola E.