Best practices for germ-free derivation and gnotobiotic zebrafish husbandry.
Best practices for germ-free derivation and gnotobiotic zebrafish husbandry.
复制标题
无菌衍生和gnotobiotic斑马鱼饲养的最佳实践。
DOI：
10.1016/bs.mcb.2016.11.005
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发表时间：
2017
影响因子：
--
通讯作者：
Guillemin K
中科院分区：
文献类型：
--
作者：
Melancon E;Gomez De La Torre Canny S;Sichel S;Kelly M;Wiles TJ;Rawls JF;Eisen JS;Guillemin K
All animals are ecosystems with resident microbial communities, referred to as microbiota, which play profound roles in host development, physiology, and evolution. Enabled by new DNA sequencing technologies, there is a burgeoning interest in animal–microbiota interactions, but dissecting the specific impacts of microbes on their hosts is experimentally challenging. Gnotobiology, the study of biological systems in which all members are known, enables precise experimental analysis of the necessity and sufficiency of microbes in animal biology by deriving animals germ-free (GF) and inoculating them with defined microbial lineages. Mammalian host models have long dominated gnotobiology, but we have recently adapted gnotobiotic approaches to the zebrafish (Danio rerio), an important aquatic model. Zebrafish offer several experimental attributes that enable rapid, large-scale gnotobiotic experimentation with high replication rates and exquisite optical resolution. Here we describe detailed protocols for three procedures that form the foundation of zebrafish gnotobiology: derivation of GF embryos, microbial association of GF animals, and long-term, GF husbandry. Our aim is to provide sufficient guidance in zebrafish gnotobiotic methodology to expand and enrich this exciting field of research.
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影响因子：
12.2
作者：
Haiser HJ;Seim KL;Balskus EP;Turnbaugh PJ
通讯作者：
Turnbaugh PJ
影响因子：
2.7
作者：
Bates, Jennifer M.;Mittge, Erika;Guillemin, Karen
通讯作者：
Guillemin, Karen
影响因子：
30.3
作者：
Bates, Jennifer M.;Akerlund, Janie;Guillemin, Karen
通讯作者：
Guillemin, Karen
影响因子：
6.4
作者：
Jemielita M;Taormina MJ;Burns AR;Hampton JS;Rolig AS;Guillemin K;Parthasarathy R
通讯作者：
Parthasarathy R
影响因子：
11
作者：
Burns, Adam R.;Stephens, W. Zac;Bohannan, Brendan J. M.
通讯作者：
Bohannan, Brendan J. M.