Growth-regulating factor 15 is required for leaf size control in Populus
Growth-regulating factor 15 is required for leaf size control in Populus
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控制杨树叶片大小需要生长调节因子 15
DOI：
10.1093/treephys/tpy107
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发表时间：
2018-10
期刊：
影响因子：
4
通讯作者：
Lu Mengzhu
中科院分区：
文献类型：
--
作者：
Zhou Houjun;Song Xueqin;Wei Kaili;Zhao Yanqiu;Jiang Cheng;Wang Jinnan;Tang Fang;Lu Mengzhu
生长调节因子(GRF)参与多种发育事件，特别是叶片发育。然而，GRFS在木本植物中的功能仍然难以捉摸。本研究对GRF15在杨树中的功能进行了研究。大多数GRFs在幼叶中优先表达。由于GRF15基因在大叶杨树中的表达水平最高，所占比例最高，因此对该基因进行了转基因分析。启动子-β-葡萄糖醛酸苷酶分析表明，GRF15在叶片展开区有表达。此外，还发现GRF15定位于细胞核中，并受miR396调控。GRF15高表达品系和显性抑制品系的叶片大小和栅栏细胞大小分别显著增加和减少。一致地，在GRF15过表达和显性抑制系中，与细胞扩展标记EXPA11同源的EXPA11a的表达分别在GRF15过表达和显性抑制系中强烈上调和下调，这在反式激活实验中进一步表现为GRF15激活EXPA11a。因此，GRF15是控制杨树叶片大小的必需基因，它主要调控杨树叶片发育过程中的细胞扩张。
Growth-regulating factors (GRFs) are involved in various developmental events, particularly leaf development. However, the functions of GRFs in woody plants remain elusive. In this study, functional characterization of GRF15 in Populus was performed. Most GRFs are preferentially expressed in young leaves. As GRF15 was expressed at the highest level and with highest ratio in Populus species with large leaves, this gene was investigated through transgenic analyses. Promotor-β-glucuronidase analysis revealed expression of GRF15 at the leaf expansion zone. Additionally, GRF15 was found to be localized in the nucleus and regulated by miR396. Leaf size and palisade cell size were significantly increased and decreased in GRF15-overexpressing and dominant repression lines, respectively. Consistently, expression of EXPA11a, a homolog of cell-expansion marker EXPA11 in Arabidopsis, was strongly upregulated and downregulated in the GRF15-overexpressing and dominant repression lines, respectively, which was further manifested by activation of EXPA11a by GRF15 in transactivation assays. Therefore, GRF15 is required for leaf size control and primarily modulates cell expansion during leaf development in Populus.
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