In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers.
In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. II. A common clonal origin for periarteriolar lymphoid sheath-associated foci and germinal centers.
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对（4-羟基-3-硝基苯基）乙酰基的初级免疫反应的原位研究。二.动脉周围淋巴鞘相关病灶和生发中心的常见克隆起源。
DOI：
10.1084/jem.176.3.679
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发表时间：
1992-09-01
期刊：
影响因子：
--
通讯作者：
Kelsoe G
中科院分区：
文献类型：
--
作者：
Jacob J;Kelsoe G
在与蛋白载体偶联的（4-羟基-3-硝基苯基）乙酰基免疫后的遗传限制性应答中，在C57 BL/6小鼠的脾脏中观察到两个不同的B细胞群。免疫后48 h，抗原结合B细胞灶沿着动脉周围淋巴鞘周围出现。这些病灶扩大，包含大量的抗体形成细胞，既不结合凝集素，花生凝集素，也不突变重排的免疫球蛋白可变区基因座。免疫后96-120 h可观察到含有花生凝集素阳性B细胞的生殖中心。尽管对免疫半抗原具有特异性，但这些B细胞不产生大量抗体，而是经历体细胞超突变和亲和力驱动选择的群体。病灶和生发中心人群均为少克隆，平均由3个或更少B淋巴细胞形成。尽管病灶（早期抗体产生）和生发中心（较高亲和力记忆细胞）B细胞群具有高度特化的作用，但对邻近病灶和生发中心中VH至D至JH连接的分析表明，这些B细胞群具有共同的克隆起源。
In the genetically restricted response that follows immunization with (4-hydroxy-3-nitrophenyl)acetyl coupled to protein carriers, two distinct populations of B cells are observed in the spleens of C57BL/6 mice. By 48 h postimmunization, foci of antigen-binding B cells appear along the periphery of the periarteriolar lymphoid sheaths. These foci expand to contain large numbers of antibody-forming cells that neither bind the lectin, peanut agglutinin, nor mutate the rearranged immunoglobulin variable region loci. Germinal centers containing peanut agglutinin-positive B cells can be observed by 96-120 h after immunization. Although specific for the immunizing hapten, these B cells do not produce substantial amounts of antibody, but are the population that undergoes somatic hypermutation and affinity-driven selection. Both focus and germinal center populations are pauciclonal, founded, on average, by three or fewer B lymphocytes. Despite the highly specialized roles of the focus (early antibody production) and germinal center (higher affinity memory cells) B cell populations, analysis of VH to D to JH joins in neighboring foci and germinal centers demonstrate that these B cell populations have a common clonal origin.
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